润滑液体注入的多孔釉质表面的生物清污性能的研究
发布时间：2018-11-03 13:52
【摘要】：目的本文主要探讨构建润滑液体注入的多孔釉质表面后,该表面对于唾液黏蛋白的吸附以及细菌生物膜形成的影响。方法制备若干尺寸为4×4×2mm3牛牙釉质片,备用。用37%的磷酸酸蚀1min后冲洗,然后随机分为四组。以1H,1H,2H,2H-全氟癸基三氯硅烷和全氟三戊胺依次处理釉质片为实验组,以去离子水处理的釉质片为对照组1,仅以全氟三戊胺处理的釉质片为对照组2以及以1H,1H,2H,2H-全氟癸基三氯硅烷处理的釉质片为对照组3。用傅里叶红外光谱仪以及原子力显微镜检测各组样品表面,并检测样品表面水接触角,其中每组测量六个样品。采用酶标仪检测各组样品表面唾液黏蛋白黏附后,用阿新蓝溶液染色后的洗脱液在595nm下的光密度值,以检测唾液黏蛋白吸附情况。用场发射扫描电子显微镜观测各组样品在4小时,24小时以及48小时后表面细菌生物膜生长情况。并用菌落形成单位计数法计算各组样品表面三个时间组的细菌菌落数,每组有六个样品。在动物实验中,以在新西兰大白兔的一侧前牙上构建的润滑液体多孔釉质表面为实验组,对侧同名牙仅以35%磷酸凝胶酸蚀为对照组。饲以高糖饮食,并于48小时后用菌斑指示剂染色,观察两组前牙的牙菌斑染色情况。结果傅里叶红外光谱和原子力显微镜显示实验组1H,1H,2H,2H-全氟癸基三氯硅烷和全氟三戊胺均吸附于多孔釉质表面,润滑液体多孔釉质表面构建成功。其中原子力显微镜下可见,实验组样品表面粗糙度明显小于三个对照组样本。对照组1、对照组2、对照组3以及实验组的水接触角结果差异有统计学意义(P0.05)。对照组1、对照组2、对照组3以及实验组的黏蛋白吸附洗脱液的光密度值结果差异有统计学意义(P0.05)。实验组样品表面在4小时后几乎无细菌生长而在3个对照组中均只有少量菌落形成。24小时后,实验组样品表面有少量稀疏的菌落形成, 而对照组样品表面有交织的细菌菌落密集分布,但仍可见样品表面。在48小时后,实验组样品表面仍然只有少量菌落分布,与24小时组相比,视野中菌落增加较少。而三个对照组样品表面均被均匀且厚的菌落密集覆盖,釉质表面完全不可见。由菌落形成单位计数结果可见,在三个时间段实验组样品表面菌落计数均少于三个对照组,差异有统计学意义(P0.05)。由新西兰大白兔体内实验可见,48小时后,实验组牙齿牙菌斑染色明显少于对照组。结论本研究构建的润滑液体多孔釉质表面可以有效抑制唾液黏蛋白的吸附以及细菌生物膜的形成。
[Abstract]:Objective to investigate the effect of the porous enamel surface implanted with lubricating fluid on the adsorption of salivary mucin and the formation of bacterial biofilm. Methods A number of 4 脳 4 脳 2mm3 bovine enamel tablets were prepared and set aside. 1min was washed with 37% phosphoric acid and then randomly divided into four groups. The enamel tablets treated with 1H ~ (-1) H ~ (2) fluorodecyl trichlorosilane and perfluorotripentylamine in turn were used as the experimental group, the enamel tablets treated with deionized water as the control group (1), only the enamel tablets treated with perfluorotripentylamine as the control group, and the enamel tablets treated The enamel treated with 2 H 2 H-perfluorodecyl trichlorosilane was the control group 3. FT-IR and AFM were used to detect the surface of each group of samples, and the water contact angle of each group of samples was measured. Six samples were measured in each group. The adhesion of salivary mucin on the surface of each group was detected by enzyme labeling instrument, and the optical density of the eluate stained with azin blue solution was measured under 595nm to detect the adsorption of salivary mucin. The growth of bacterial biofilm was observed by field emission scanning electron microscope (SEM) after 4 hours, 24 hours and 48 hours. The number of bacterial colonies in three time groups of each group was calculated by colony forming unit count method, and there were six samples in each group. In animal experiment, the porous enamel surface of lubricated liquid was constructed on one front tooth of New Zealand white rabbit as experimental group, and the control group was only 35% phosphoric acid etching on the opposite side of homonym teeth. The dental plaque staining of the anterior teeth of the two groups was observed after 48 hours of high glucose diet and stain with plaque indicator. Results the results of Fourier transform infrared spectroscopy and atomic force microscope showed that the experimental group (1H ~ (-1) H ~ (2) H _ (2) F _ (3) H _ (3) Chlorosilane and PFC _ (3) were adsorbed on the surface of porous enamel. The surface roughness of the experimental group was obviously less than that of the three control group samples under atomic force microscope (AFM). The water contact angle of control group 1, control group 2, control group 3 and experimental group were significantly different (P0.05). In control group 1, control group 2, control group 3 and experimental group of mucin adsorption eluate optical density value difference was statistically significant (P0.05). There was almost no bacterial growth on the surface of the samples in the experimental group after 4 hours, but only a small number of colonies were formed in the three control groups. 24 hours later, a small number of sparse colonies formed on the surface of the samples in the experimental group. In the control group, there were interlaced bacterial colonies on the surface of the sample, but the surface of the sample was still visible. After 48 hours, there were only a few colonies on the surface of the samples in the experimental group, compared with the 24 hour group, the colony increased less in the visual field. The surface of the three control groups was covered with uniform and thick colony, and the enamel surface was completely invisible. From the results of colony formation unit count, the surface colony count of the experimental group was less than that of the three control groups in three time periods, the difference was statistically significant (P0.05). After 48 hours, dental plaque staining in the experimental group was significantly less than that in the control group. Conclusion the lubricated porous enamel surface can effectively inhibit the adsorption of salivary mucin and the formation of bacterial biofilm.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783
本文编号：2307984
[Abstract]:Objective to investigate the effect of the porous enamel surface implanted with lubricating fluid on the adsorption of salivary mucin and the formation of bacterial biofilm. Methods A number of 4 脳 4 脳 2mm3 bovine enamel tablets were prepared and set aside. 1min was washed with 37% phosphoric acid and then randomly divided into four groups. The enamel tablets treated with 1H ~ (-1) H ~ (2) fluorodecyl trichlorosilane and perfluorotripentylamine in turn were used as the experimental group, the enamel tablets treated with deionized water as the control group (1), only the enamel tablets treated with perfluorotripentylamine as the control group, and the enamel tablets treated The enamel treated with 2 H 2 H-perfluorodecyl trichlorosilane was the control group 3. FT-IR and AFM were used to detect the surface of each group of samples, and the water contact angle of each group of samples was measured. Six samples were measured in each group. The adhesion of salivary mucin on the surface of each group was detected by enzyme labeling instrument, and the optical density of the eluate stained with azin blue solution was measured under 595nm to detect the adsorption of salivary mucin. The growth of bacterial biofilm was observed by field emission scanning electron microscope (SEM) after 4 hours, 24 hours and 48 hours. The number of bacterial colonies in three time groups of each group was calculated by colony forming unit count method, and there were six samples in each group. In animal experiment, the porous enamel surface of lubricated liquid was constructed on one front tooth of New Zealand white rabbit as experimental group, and the control group was only 35% phosphoric acid etching on the opposite side of homonym teeth. The dental plaque staining of the anterior teeth of the two groups was observed after 48 hours of high glucose diet and stain with plaque indicator. Results the results of Fourier transform infrared spectroscopy and atomic force microscope showed that the experimental group (1H ~ (-1) H ~ (2) H _ (2) F _ (3) H _ (3) Chlorosilane and PFC _ (3) were adsorbed on the surface of porous enamel. The surface roughness of the experimental group was obviously less than that of the three control group samples under atomic force microscope (AFM). The water contact angle of control group 1, control group 2, control group 3 and experimental group were significantly different (P0.05). In control group 1, control group 2, control group 3 and experimental group of mucin adsorption eluate optical density value difference was statistically significant (P0.05). There was almost no bacterial growth on the surface of the samples in the experimental group after 4 hours, but only a small number of colonies were formed in the three control groups. 24 hours later, a small number of sparse colonies formed on the surface of the samples in the experimental group. In the control group, there were interlaced bacterial colonies on the surface of the sample, but the surface of the sample was still visible. After 48 hours, there were only a few colonies on the surface of the samples in the experimental group, compared with the 24 hour group, the colony increased less in the visual field. The surface of the three control groups was covered with uniform and thick colony, and the enamel surface was completely invisible. From the results of colony formation unit count, the surface colony count of the experimental group was less than that of the three control groups in three time periods, the difference was statistically significant (P0.05). After 48 hours, dental plaque staining in the experimental group was significantly less than that in the control group. Conclusion the lubricated porous enamel surface can effectively inhibit the adsorption of salivary mucin and the formation of bacterial biofilm.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783
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