GSK-3β对正畸牙齿移动的影响

发布时间：2018-11-03 17:24

【摘要】：目的:观察GSK-3β对正畸牙齿移动距离的影响。方法:分别选30只野生C57小鼠和20只GSK-3β基因敲除C57小鼠。野生型和基因敲除型各20只,正畸加力3、5、7、14 d(5只/组)后处死,取材固定上颌骨扫描Micro CT测量牙齿移动距离,冰冻切片免疫荧光染色观察破骨情况。野生型10只腹腔注射LiCl(隔天100 ng/ml)加力3 d,7 d处死后扫描Micro CT。结果:与野生型组同期结果相比,GSK-3β基因敲除小鼠牙齿移动距离降低(P0.05),压力侧破骨细胞减少(P0.05);基因敲除组与野生型注射LiCl组牙齿移动距离无统计学差异。结论:GSK-3β可以影响破骨细胞形成从而影响正畸牙齿移动。
[Abstract]:Objective: to observe the effect of GSK-3 尾 on the movement distance of orthodontic teeth. Methods: 30 wild C57 mice and 20 GSK-3 尾 knockout mice were selected. Wild type and gene knockout type (n = 20 each) were killed after orthodontic treatment (n = 20) for 14 days (n = 5). Maxillary bone was fixed with scanning Micro CT to measure the distance of tooth movement. The osteoclasts were observed by immunofluorescence staining in frozen sections. Ten wild-type mice were killed by intraperitoneal injection of LiCl (100 ng/ml every other day) for 3 days and 7 days, then Micro CT. was scanned. Results: compared with the wild type group, the tooth movement distance of GSK-3 尾 gene knockout mice decreased (P0.05), and the osteoclasts of pressure side decreased (P0.05). There was no significant difference in tooth movement distance between gene knockout group and wild type injection LiCl group. Conclusion: GSK-3 尾 can affect osteoclast formation and orthodontic tooth movement.
【作者单位】：军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔正畸科;军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学组织工程中心;
【基金】：国家自然科学基金(编号:81171001)
【分类号】：R783.5

【相似文献】