地塞米松对成牙骨质细胞粘附和矿化功能的调节作用

发布时间：2018-11-05 19:36

【摘要】：目的：牙根吸收是正畸治疗中的常见并发症，是牙根吸收与修复之间失衡所致。牙骨质是覆盖在牙根表面的一层矿化结缔组织，是牙周膜附着的重要结构。炎症或异常机械力刺激会激活破牙骨质前体细胞的分化、成熟，从而引起牙骨质的破坏，甚至波及牙本质而导致不可逆性牙根吸收，在这一过程中起中心作用的是成牙骨质细胞。成牙骨质细胞是牙周组织中牙骨质形成的重要功能细胞，在牙根形成、牙骨质修复过程中起着关键性作用，其主要功能是分泌新生的牙骨质样组织，形成牙骨质基质，对已经吸收的牙根表面进行修复。地塞米松是一种类固醇类激素，能增强组织的矿化能力，刺激成骨细胞表达矿化相关基因，在成骨过程中有着重要的地位，但国内外对于地塞米松作用于成牙骨质细胞后的分子生物学研究很少。因此，本研究的目的是探讨地塞米松对小鼠成牙骨质细胞株OCCM-30增殖活性、细胞粘附和矿化功能的影响，以初步了解地塞米松在成牙骨质细胞修复过程中所起的作用，从而为牙骨质再生提供理论依据。方法：本研究将地塞米松作用于成牙骨质细胞株OCCM-30，利用CCK-8法、细胞内ALP活性检测、茜素红染色鉴定矿化结节以及Q-PCR检测等方法，对不同浓度地塞米松作用下的，成牙骨质细胞的增殖能力、粘附及矿化能力进行初步研究。结果： 1．地塞米松能够抑制成牙骨质细胞的增殖，且高浓度者（1×10-4mol/L）效果强度高于低浓度者。 2．成牙骨质细胞具有ALP活性，和对照组相比，实验组ALP活性明显增高。 3．茜素红染色结果显示，实验组与对照组相比，矿化结节的数量呈明显增多趋势，，其中以1×10-7mol/L浓度组增加最为明显。 4．Q-PCR结果显示，细胞粘附功能相关基因Cadherin-11和N-CadherinmRNA的表达随地塞米松浓度的增大而增加。 5．Q-PCR结果显示，矿化相关基因ALP、BSP、OCN、Rumx-2mRNA的表达与对照组相比明显增高。结论：地塞米松可以增加成牙骨质细胞的粘附及矿化功能，并可能由此影响牙根吸收和修复的过程。
[Abstract]:Objective: root resorption is a common complication in orthodontic treatment. Cementum is a layer of mineralized connective tissue covering the root surface and an important structure of periodontal ligament attachment. Inflammation or abnormal mechanical stimulation can activate the differentiation and maturation of osteoclastic precursor cells, which can lead to the destruction of cementum, and even to dentin, leading to irreversible root resorption. Cementoblasts play a central role in this process. Cementoblasts are important functional cells in the formation of cementum in periodontal tissue. They play a key role in root formation and cementum repair. Their main function is to secrete new cementoid tissue to form cemental matrix. Repair the absorbed root surface. Dexamethasone is a steroid hormone that enhances tissue mineralization and stimulates osteoblasts to express mineralization-related genes, which plays an important role in osteogenesis. However, there are few studies on molecular biology of dexamethasone acting on cementoblasts at home and abroad. Therefore, the purpose of this study was to investigate the effects of dexamethasone on OCCM-30 proliferation, cell adhesion and mineralization in mouse cementoblast cell lines, and to explore the role of dexamethasone in the process of cementoblasts repair. So as to provide theoretical basis for cementum regeneration. Methods: in this study, dexamethasone was applied to cementoblast cell line OCCM-30, by CCK-8 assay, intracellular ALP activity detection, alizarin red staining for the identification of mineralized nodules and Q-PCR detection. The effects of dexamethasone on the proliferation, adhesion and mineralization of cementoblasts were studied. Results: 1. Dexamethasone could inhibit the proliferation of cementoblasts, and the effect of dexamethasone at high concentration (1 脳 10-4mol/L) was higher than that of low concentration. 2. The activity of ALP was found in cementoblasts, and the activity of ALP in experimental group was significantly higher than that in control group. 3.The results of alizarin red staining showed that the number of mineralized nodules in the experimental group was obviously increased compared with that in the control group, especially in the 1 脳 10-7mol/L group. 4.Q-PCR results showed that the expression of Cadherin-11 and N-CadherinmRNA increased with the increase of dexamethasone concentration. 5.Q-PCR results showed that the expression of mineralization-related gene ALP,BSP,OCN,Rumx-2mRNA was significantly higher than that of the control group. Conclusion: dexamethasone can increase the adhesion and mineralization of cementoblasts and may affect the process of root resorption and repair.
【学位授予单位】：广州医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5

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