疱疹病毒与牙周致病菌在逆行性牙髓炎中的相关性研究
发布时间：2018-11-07 15:37
【摘要】：目的：探讨逆行性牙髓炎疱疹病毒与五种牙周致病菌之间的关系以及逆行性牙髓炎疾病的致病危险因素，初步了解疱疹病毒与牙周致病菌之间相互作用的机制，，为逆行性牙髓炎及重度牙周病损的临床诊治提供科学依据。 方法：以前期研究对38例逆行性牙髓炎牙髓组织和龈下菌斑以及30例牙周健康受试者的龈下菌斑样本检测五种牙周致病菌，伴放线聚集杆菌(Aggregatibacter actinomycetemcomitans, A.a.)，牙龈卟啉单胞菌(Porphyromonas gingivalis, P.g.）,中间普氏菌(Prevotella intermedia, P.i),福赛坦氏菌(Tannerella forsythensis, T.f.)，齿垢密螺旋体(Treponema denticola,T.d.)，以及疱疹病毒人巨细胞病毒（Human cytomegalovirus, HCMV）和EB病毒1型（Epstein-Barr virus-1, EBV-1）所获得的数据为基础，本研究整理前期数据，使用SPSS13.0软件包，应用行×列X2检验比较各牙周致病菌在EBV-1/HCMV分层后的检出率，应用Spearman秩相关分析其相关性。应用两独立样本非参数秩和检验Mann-Whitney U检验方法比较各牙周致病菌在EBV-1/HCMV分层后的构成比差异。应用行×列X2检验比较不同牙周致病菌检出的种类数时疱疹病毒的检出率。应用单变量逻辑回归分析龈下感染原对逆行性牙髓炎的相对危险度，以及五种牙周致病菌构成比对EBV-1/HCMV的相对危险度。 结果：1.逆行性牙髓炎牙髓组织中EBV-1阳性时P.g.和T.f.的检出率高于EBV-1阴性时的检出率。逆行性牙髓炎牙髓组织和龈下菌斑中EBV-1阳性时T.f.的构成比高于EBV-1阴性时的T.f.的构成比。T.f.与EBV-1显著相关，其比值比为1.10。 2.逆行性牙髓炎牙髓组织中HCMV阳性时P.i.、T.f.、T.d.的构成比高于HCMV阴性时的构成比，且T.f在两组中的差异具有统计学意义（P′=0.0490.05）。在逆行性牙髓炎龈下菌斑中HCMV阳性时P.g.、T.f.的构成比高于HCMV阴性时的构成比。T.f.与HCMV相关性具有统计学意义，其比值比为1.06。 3.检出3～5种牙周致病菌的逆行性牙髓炎牙髓样本中，EBV-1的阳性率显著高于未检出牙周致病菌的样本，差异有统计学意义（х2=5.657，P=0.043）,且疱疹病毒阳性样本中感染3～5种牙周致病菌的样本所占比例高于感染两种及两种以下致病菌样本的比例。 4.逆行性牙髓炎的相对危险因素为P.g.、P.i.、T.f.、T.d.和EBV-1、HCMV，其比值比分别为3.95,1.37,1.14,4.09,3.40和7.28。 结论：P.g.、P.i.、T.f.、T.d.和EBV-1、HCMV是逆行性牙髓炎疾病发病的危险因素。EBV-1和HCMV与牙周致病菌之间可能存在相互作用，尤其是与P.g.和T.f.存在较为密切的交互影响。同时，多种细菌混合感染可能以联合的形式与疱疹病毒相互作用，从而参与疾病的进程。但是这五种牙周致病菌与疱疹病毒EBV-1和HCMV之间的相关性不强，提示可能还存在其他病原微生物或多种病原微生物联合的形式与疱疹病毒相互影响，参与到逆行性牙髓炎疾病的致病机制中。
[Abstract]:Objective: to investigate the relationship between herpesvirus and five periodontal pathogens in retrograde pulpitis and the risk factors of retrograde pulpitis, and to understand the mechanism of the interaction between herpesvirus and periodontal pathogenic bacteria. To provide scientific basis for clinical diagnosis and treatment of retrograde pulpitis and severe periodontal disease. Methods: the subgingival plaque samples of 38 patients with retrograde pulpitis and subgingival plaque and 30 healthy subjects were studied for five kinds of periodontal pathogenic bacteria (Aggregatibacter actinomycetemcomitans, A. a.). Porphyromonas gingivalis (Porphyromonas gingivalis, P.g., (Prevotella intermedia, P.I, (Tannerella forsythensis, T.f., Treponema denticola,T.d., Based on the data obtained from herpesvirus human cytomegalovirus (Human cytomegalovirus, HCMV) and EB virus type 1 (Epstein-Barr virus-1, EBV-1), the preliminary data were collated and SPSS13.0 software package was used. The detection rate of periodontal pathogenic bacteria after EBV-1/HCMV stratification was compared by X column X 2 test and Spearman rank correlation analysis was used. The nonparametric rank sum test (Mann-Whitney U test) was used to compare the composition ratio of periodontal pathogenic bacteria after EBV-1/HCMV stratification. The detection rate of herpesvirus in different periodontal pathogenic bacteria was compared by X column X 2 test. The relative risk of subgingival infection to retrograde pulpitis and the relative risk of EBV-1/HCMV to five periodontal pathogens were analyzed by univariate logistic regression analysis. Results: 1. When EBV-1 was positive in pulp tissue of retrograde pulpitis, P.g. And T. F. The positive rate of EBV-1 was higher than that of EBV-1 negative. When EBV-1 was positive in pulp tissue and subgingival plaque of retrograde pulpitis, T. f. The ratio of T. f. was higher than that of T. f. when EBV-1 was negative. T. F. There was significant correlation with EBV-1, the ratio was 1. 10. 2. When HCMV was positive in pulp tissue of retrograde pulpitis, P. I. T. F. T. D. The ratio of T. f in the two groups was higher than that in the HCMV negative group (P < 0. 049. 05), and the difference between the two groups was statistically significant (P < 0. 049. 05). When HCMV was positive in subgingival plaque of retrograde pulpitis, P. T. F. The correlation with HCMV was statistically significant, and the ratio was 1. 06. 3. The positive rate of EBV-1 was significantly higher in the pulp samples of retrograde pulpitis with 35 periodontal pathogenic bacteria than that in the undetectable samples, and the difference was statistically significant (P 0.043). The proportion of 35 periodontal pathogens infected with herpesvirus positive samples was higher than that of the samples infected with two or less kinds of pathogenic bacteria. 4. The relative risk factors of retrograde pulpitis were P. The ratio to EBV-1,HCMV, is 3.95 ~ 1.37 ~ 1.144.09 ~ 3.40 and 7.28 respectively. Conclusion: P.g. P. I. C. T. F. T. D. EBV-1 and HCMV may interact with periodontal pathogens, especially P.g. And T. F. There is close interaction. At the same time, multiple bacterial mixed infections may interact with herpesvirus in the form of union, thus participating in the disease process. However, the correlation between these five periodontal pathogens and herpesvirus EBV-1 and HCMV is not strong, suggesting that there may be other pathogenic microorganisms or a combination of several pathogenic microorganisms and the interaction between herpesvirus and other pathogenic microorganisms. Participate in the pathogenesis of retrograde pulpitis.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.31
本文编号：2316798
[Abstract]:Objective: to investigate the relationship between herpesvirus and five periodontal pathogens in retrograde pulpitis and the risk factors of retrograde pulpitis, and to understand the mechanism of the interaction between herpesvirus and periodontal pathogenic bacteria. To provide scientific basis for clinical diagnosis and treatment of retrograde pulpitis and severe periodontal disease. Methods: the subgingival plaque samples of 38 patients with retrograde pulpitis and subgingival plaque and 30 healthy subjects were studied for five kinds of periodontal pathogenic bacteria (Aggregatibacter actinomycetemcomitans, A. a.). Porphyromonas gingivalis (Porphyromonas gingivalis, P.g., (Prevotella intermedia, P.I, (Tannerella forsythensis, T.f., Treponema denticola,T.d., Based on the data obtained from herpesvirus human cytomegalovirus (Human cytomegalovirus, HCMV) and EB virus type 1 (Epstein-Barr virus-1, EBV-1), the preliminary data were collated and SPSS13.0 software package was used. The detection rate of periodontal pathogenic bacteria after EBV-1/HCMV stratification was compared by X column X 2 test and Spearman rank correlation analysis was used. The nonparametric rank sum test (Mann-Whitney U test) was used to compare the composition ratio of periodontal pathogenic bacteria after EBV-1/HCMV stratification. The detection rate of herpesvirus in different periodontal pathogenic bacteria was compared by X column X 2 test. The relative risk of subgingival infection to retrograde pulpitis and the relative risk of EBV-1/HCMV to five periodontal pathogens were analyzed by univariate logistic regression analysis. Results: 1. When EBV-1 was positive in pulp tissue of retrograde pulpitis, P.g. And T. F. The positive rate of EBV-1 was higher than that of EBV-1 negative. When EBV-1 was positive in pulp tissue and subgingival plaque of retrograde pulpitis, T. f. The ratio of T. f. was higher than that of T. f. when EBV-1 was negative. T. F. There was significant correlation with EBV-1, the ratio was 1. 10. 2. When HCMV was positive in pulp tissue of retrograde pulpitis, P. I. T. F. T. D. The ratio of T. f in the two groups was higher than that in the HCMV negative group (P < 0. 049. 05), and the difference between the two groups was statistically significant (P < 0. 049. 05). When HCMV was positive in subgingival plaque of retrograde pulpitis, P. T. F. The correlation with HCMV was statistically significant, and the ratio was 1. 06. 3. The positive rate of EBV-1 was significantly higher in the pulp samples of retrograde pulpitis with 35 periodontal pathogenic bacteria than that in the undetectable samples, and the difference was statistically significant (P 0.043). The proportion of 35 periodontal pathogens infected with herpesvirus positive samples was higher than that of the samples infected with two or less kinds of pathogenic bacteria. 4. The relative risk factors of retrograde pulpitis were P. The ratio to EBV-1,HCMV, is 3.95 ~ 1.37 ~ 1.144.09 ~ 3.40 and 7.28 respectively. Conclusion: P.g. P. I. C. T. F. T. D. EBV-1 and HCMV may interact with periodontal pathogens, especially P.g. And T. F. There is close interaction. At the same time, multiple bacterial mixed infections may interact with herpesvirus in the form of union, thus participating in the disease process. However, the correlation between these five periodontal pathogens and herpesvirus EBV-1 and HCMV is not strong, suggesting that there may be other pathogenic microorganisms or a combination of several pathogenic microorganisms and the interaction between herpesvirus and other pathogenic microorganisms. Participate in the pathogenesis of retrograde pulpitis.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.31
【参考文献】
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1 钟钰;高碧云;黄燕琳;陈文霞;;红色复合体与逆行性牙髓炎的相关性研究[J];口腔生物医学;2012年01期
本文编号：2316798
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