不同管径钛纳米管对成纤维细胞增殖、伸展和胶原分泌功能的影响

发布时间：2018-11-11 19:18

【摘要】：目的:研究不同管径Ti-TiO_2纳米管对成纤维细胞增殖、伸展和胶原分泌功能的影响。方法:以1、5、10、20 V电压阳极氧化制备不同管径Ti-TiO_2纳米管试件,试件表面培养成纤维细胞,MTT法检测细胞增殖,扫描电镜观察形态,天狼星红苦味酸染色检测细胞胶原分泌功能。结果:1、5、10、20 V制备的纳米管径依次为15、30、50和100 nm。细胞在抛光表面的增殖均高于纳米管表面;第5天时,100 nm纳米管表面细胞的增殖显著高于其他(P0.01)。第3天时,抛光表面细胞呈典型长梭型,纳米管表面细胞伪足明显。100 nm纳米管表面细胞的试件胶原分泌功能最大(P0.05)。结论:100 nm纳米管对细胞增殖的抑制作用较小并能促进细胞的胶原纤维分泌功能。
[Abstract]:Aim: to study the effects of Ti-TiO_2 nanotubes with different diameters on the proliferation, extension and collagen secretion of fibroblasts. Methods: Ti-TiO_2 nanotube specimens with different diameters were prepared by anodic oxidation at a voltage of 1020 V. The fibroblasts were cultured on the surface of the specimens. The proliferation of the cells was detected by MTT, and the morphology was observed by scanning electron microscope (SEM). Sirius red picric acid staining was used to detect the function of collagen secretion. Results: 1 the diameter of nanotubes prepared at 1020 V was 1550 and 100 nm., respectively. The proliferation of the cells on the polished surface was higher than that on the surface of the nanotubes, and on the 5th day, the proliferation of the cells on the surface of 100 nm nanotubes was significantly higher than that on the other surfaces (P0.01). On the third day, the polished surface cells showed typical long spindle type, and the pseudopodia of the nanotube surface cells were obvious. The collagen secretion function of the 100 nm nanotube surface cells was the largest (P0.05). Conclusion: 100 nm nanotubes have little inhibitory effect on cell proliferation and promote the secretion of collagen fibers.
【作者单位】：株洲市中心医院口腔科;
【基金】：湖南省2013年度医药卫生科研计划课题(编号:C2013-051)
【分类号】：R783.6

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