粪肠球菌体外根尖生物膜模型的建立及形态学研究
发布时间：2018-11-18 09:46
【摘要】：目的通过体外实验建立根尖生物膜模型,观察粪肠球菌根尖生物膜模型的形态及结构。方法选取人新鲜拔除的单根离体牙24颗,随机分为8组,每组3颗,浸泡于粪肠球菌ATCC 29212菌悬液中,分别于1、2、7 d建立根尖生物膜模型。使用扫描电子显微镜(SEM)观察生物膜形成过程;使用碘化丙啶(PI)和刀豆球蛋白A异硫氰酸荧光素(ConA-FITC)对生物膜细菌及细胞外基质进行双染色,通过激光共聚焦扫描电子显微镜(CLSM)观察其形态和结构;计算生物膜覆盖率。结果随时间的增加,根尖样本表面细菌覆盖面积增加,7 d组与1、2 d组间的差异存在统计学意义(P0.05),1 d与2 d组间的差异无统计学意义(P0.05)。7 d组形成生物膜结构,其样本表面的覆盖率为17.23%±1.52%,样本表面有大量细菌附着,细菌被细胞外基质包绕,并呈群落分布。生物膜中的细菌被PI染成红色,细胞外基质被ConA-FITC染成绿色,可见多层次的空间结构以及亲水性通道。结论粪肠球菌根尖生物膜于培养7 d时形成完整的生物膜结构,由细菌群落及其周围大量的不定型基质组成。
[Abstract]:Objective to establish the root tip biofilm model in vitro and observe the morphology and structure of Enterococcus faecalis root tip biofilm model. Methods A total of 24 isolated teeth were randomly divided into 8 groups, 3 in each group, soaked in ATCC 29212 suspension of Enterococcus faecalis. The model of biofilm was established at 1, 2 and 7 days, respectively. The process of biofilm formation was observed by scanning electron microscope (SEM). The biofilm bacteria and extracellular matrix were double stained with pyridine iodide (PI) and concanavalin A isothiocyanate (ConA-FITC). The morphology and structure of biofilm were observed by confocal scanning electron microscopy (CLSM). The biofilm coverage was calculated. Results with the increase of time, the bacterial coverage area of root tip samples increased, and the difference between 7 d group and 1 2 d group was statistically significant (P0.05). There was no significant difference between the 1 d and 2 d groups (P0.05). The biofilm structure was formed in the 7th day group. The coverage of the sample surface was 17.23% 卤1.52%. A large number of bacteria were attached to the sample surface and the bacteria were surrounded by extracellular matrix. And distributed in community. The bacteria in the biofilm were stained red by PI, and the extracellular matrix was stained green by ConA-FITC. Conclusion the biofilm of Enterococcus faecalis root tip formed a complete biofilm structure after 7 days of culture and was composed of a large number of amorphous substrates around the bacterial community.
【作者单位】： 首都医科大学附属北京口腔医院牙体牙髓科;
【基金】：国家自然科学基金面上项目资助项目(81170952) 首都卫生发展科研专项基金资助项目(首发2011-2014-05)
【分类号】：R781.05
[Abstract]:Objective to establish the root tip biofilm model in vitro and observe the morphology and structure of Enterococcus faecalis root tip biofilm model. Methods A total of 24 isolated teeth were randomly divided into 8 groups, 3 in each group, soaked in ATCC 29212 suspension of Enterococcus faecalis. The model of biofilm was established at 1, 2 and 7 days, respectively. The process of biofilm formation was observed by scanning electron microscope (SEM). The biofilm bacteria and extracellular matrix were double stained with pyridine iodide (PI) and concanavalin A isothiocyanate (ConA-FITC). The morphology and structure of biofilm were observed by confocal scanning electron microscopy (CLSM). The biofilm coverage was calculated. Results with the increase of time, the bacterial coverage area of root tip samples increased, and the difference between 7 d group and 1 2 d group was statistically significant (P0.05). There was no significant difference between the 1 d and 2 d groups (P0.05). The biofilm structure was formed in the 7th day group. The coverage of the sample surface was 17.23% 卤1.52%. A large number of bacteria were attached to the sample surface and the bacteria were surrounded by extracellular matrix. And distributed in community. The bacteria in the biofilm were stained red by PI, and the extracellular matrix was stained green by ConA-FITC. Conclusion the biofilm of Enterococcus faecalis root tip formed a complete biofilm structure after 7 days of culture and was composed of a large number of amorphous substrates around the bacterial community.
【作者单位】： 首都医科大学附属北京口腔医院牙体牙髓科;
【基金】：国家自然科学基金面上项目资助项目(81170952) 首都卫生发展科研专项基金资助项目(首发2011-2014-05)
【分类号】：R781.05
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【共引文献】
相关期刊论文 前9条
1 宁杨;凌均h，
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