Er:YAG激光对2型糖尿病相关性牙周炎患者龈下菌群动态变化的研究

发布时间：2018-11-22 08:35

【摘要】：目的动态观察2型糖尿病相关性牙周炎患者经Er:YAG激光治疗前后龈下菌群的变化,并与不伴全身疾病的慢性牙周炎龈下菌群进行比较。方法收集11例2型糖尿病相关性牙周炎患者的13对患牙(26个位点)作为试验组,分别进行Er:YAG激光治疗和超声治疗,采集治疗前及治疗后1、3个月的龈下菌斑;同时收集11例牙周状况相近的不伴全身疾病的中重度慢性牙周炎患者13个位点的龈下菌斑作为对照组,分析试验组是否有菌群种类变化。提取龈下菌斑DNA,进行变性梯度凝胶电泳分离及条带回收测序。结果试验组与对照组患牙龈下菌斑的优势致病菌存在差异,分别为中间普雷沃菌和福赛斯坦纳菌。激光组治疗前后龈下菌群构成也发生改变,治疗后1个月,有的条带表达减弱或消失,并有新条带出现;测序结果表明,新出现的条带为放线菌的一种,减弱、消失的为福赛斯坦纳菌。结论 Er:YAG激光治疗前后龈下菌群的构成发生变化,治疗后1个月是关键时期;治疗后3个月,激光治疗在阻止细菌再定植方面可能更具优势。
[Abstract]:Objective to observe the changes of subgingival flora in patients with type 2 diabetes mellitus associated periodontitis before and after Er:YAG laser treatment and compare it with that of chronic periodontitis without systemic disease. Methods Thirteen pairs of teeth (26 loci) of 11 patients with type 2 diabetes mellitus associated periodontitis were selected as experimental group. The subgingival plaque was collected before treatment and 1 and 3 months after treatment with Er:YAG laser and ultrasound respectively. At the same time, subgingival plaque at 13 loci in 11 patients with moderate and severe chronic periodontitis without systemic disease were collected as control group. Subgingival plaque DNA, was isolated by denaturing gradient gel electrophoresis and sequenced. Results the dominant pathogens of subgingival plaque in the test group and the control group were Prevoc intermediate and Fossestana respectively. The composition of subgingival flora also changed before and after treatment in laser group. After 1 month of treatment, some bands were weakened or disappeared, and some new bands appeared. The sequencing results showed that the new band was a species of actinomycetes, weakened and disappeared. Conclusion the composition of subgingival flora changes before and after Er:YAG laser treatment, one month after treatment is the key period, and 3 months after treatment, laser treatment may have more advantages in preventing bacterial recolonization.
【作者单位】：中国人民解放军总医院口腔医学研究所;航空总医院口腔科;口腔疾病研究国家重点实验室华西口腔医院(四川大学);
【基金】：国家自然科学基金资助项目(81271145) 四川省科技支撑计划基金资助项目(2011FZO199)
【分类号】：R781.42;R587.1

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