变形链球菌与远缘链球菌基因型在维吾尔族儿童口腔中的分布及其与重型婴幼儿龋相关性研究

发布时间：2018-11-23 17:57

【摘要】：目的：调查致龋相关变形链球菌(Mutans Streptococci, MS)在喀什封闭维吾尔族不同龋敏感儿童口腔菌斑中基因型分布状况,初探其与低龄婴幼儿龋(EarlyChildhood Caries, ECC)相关性,为儿童龋病病因学研究提供依据。方法：以24～71个月龄维吾尔族封闭人群儿童为研究对象,选符合取样标准的32例重度婴幼儿龋患儿(S-ECC组),32例无龋儿童(CF组),收集口腔菌斑样本行选择性细菌培养,根据形态学特征分离并分子生物学方法进一步鉴定变异链球菌(Streptococcus mutans,Sm)及远缘链球菌(Streptococcus sobrinus, Ss),利用随机引物聚合酶链反应(Arbitraryprimed-PCR, AP-PCR)对两种人类致龋相关变异链球菌属临床分离株基因组DNA行基因型分析。结果：两组儿童Sm检出率为82.8%(53/64), Ss检出率为29.7%(19/64), Sm及Ss检出率在两组间差异无统计学意义(P0.05)。SECC及CF组个体Sm基因型分别为1～6种及1～5种,Ss基因型为1～3种。S-ECC组中携带一种以上Sm基因型的个体占75%,携带一种以上Ss基因型的个体占15.7%；CF组携带一种以上Sm基因型的个体占59.4%,携带一种以上Ss基因型的个体占12.5%。S-ECC变形链球菌基因多态性显著高于CF组(P0.05)。Sm基因型数与dmft呈正相关(r=0.439, P0.05)。Logistic回归分析Sm及Ss基因多态性与不同龋敏感性关系,发现Sm基因多态性对疾病的发生影响是有统计学意义的(B=0.4040, P0.05=。结论：Sm, Ss在封闭维吾尔族不同龋敏感儿童菌斑检出率无明显差异,Sm, Ss临床菌株间存在基因多态性且SECC组基因型数显著高于CF组,个体携带Sm基因型种类数与其致龋性之间存在相关性而与携带Ss基因型数无明显相关性。个体携带Sm基因型越多,可能会增加患龋危险。
[Abstract]:Objective: to investigate the genotypic distribution of cariogenic streptococcus mutans (Mutans Streptococci, MS) in oral plaque of Uygur children with different caries sensitivity in Kashi, and to explore its correlation with (EarlyChildhood Caries, ECC) in young children with caries. To provide the basis for the study of the etiology of caries in children. Methods: a total of 32 children with severe caries (S-ECC group) and 32 children without caries (CF group) were enrolled in this study. Oral plaque samples were collected for selective bacterial culture and further identification of Streptococcus mutans (Streptococcus mutans,Sm) and Streptococcus distalis (Streptococcus sobrinus, Ss),) by molecular biological methods. Genomic DNA of two clinical isolates of Streptococcus mutans were genotyped by random primer polymerase chain reaction (Arbitraryprimed-PCR, AP-PCR). Results: the detection rate of Sm in the two groups was 82.8% (53 / 64), Ss was 29.7%). There was no significant difference in the detection rate of Sm and Ss between the two groups (P0.05) in the). SECC and CF groups, there were 6 Sm genotypes and 5 Sm genotypes, respectively. In S-ECC group, 75 individuals carried more than one Sm genotype, and 15.7individuals carrying more than one Ss genotype. The percentage of individuals with more than one Sm genotype in CF group was 59.4%. The genetic polymorphisms of Streptococcus mutans in 12.5%.S-ECC with more than one Ss genotype were significantly higher than those in CF group (P0.05). There was a positive correlation between the number of). Sm genotypes and dmft (r = 0.439, P < 0.05). P0.05). Logistic regression analysis showed that the polymorphism of Sm and Ss gene was associated with different caries susceptibility, and the influence of Sm gene polymorphism on the occurrence of the disease was statistically significant (BX 0.4040, P0.05 0. 05). Conclusion there was no significant difference in the detection rate of plaque in Uygur children with different caries sensitivity by: Sm, Ss. There was no significant difference in gene polymorphism among clinical strains of, Sm, Ss and the number of genotypes in SECC group was significantly higher than that in CF group. There was a correlation between the number of genotypes carrying Sm and caries, but not with the number of genotypes carrying Ss. The more individuals carry Sm genotypes, the higher the risk of caries may be.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R788.1

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