特异性沉默α-catulin基因的表达对舌鳞状细胞癌TSCCA细胞增殖和凋亡的影响

发布时间：2018-11-25 21:22

【摘要】：目的:探讨α-catulin基因对人舌鳞状细胞癌(TSCC)TSCCA细胞增殖和凋亡的影响,阐明α-catulin在TSCCA细胞生物学行为中的作用。方法:取对数生长期的TSCCA细胞,分为α-catulin-siRNA转染组、空质粒转染组和空白对照组。RT-PCR法检测各组TSCCA细胞中α-catulin mRNA相对表达水平;Western blotting法检测各组TSCCA细胞中α-catulin蛋白的相对表达水平,MTT法检测细胞生长抑制率,流式细胞术检测各组TSCCA细胞凋亡率。结果:RT-PCR检测,与空质粒转染组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞中α-catulin mRNA相对表达水平降低(P0.01),且随着时间的相对延长,α-catulin mRNA相对表达水平逐渐降低,72h时其相对表达水平低于24和48h时(P0.01)。Western blotting法检测,与空质粒转染组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞中α-catulin蛋白相对表达水平降低(P0.05)。MTT法检测,与空质粒转染组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞生长抑制率升高(P0.001)。流式细胞术检测,与空质粒对照组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞凋亡率升高(P0.001)。结论:特异性沉默α-catulin基因的表达可抑制TSCC的TSCCA细胞的增殖,促进其凋亡。
[Abstract]:Aim: to investigate the effect of 伪-catulin gene on proliferation and apoptosis of human tongue squamous cell carcinoma (TSCC) TSCCA) cells and to elucidate the role of 伪-catulin in the biological behavior of TSCCA cells. Methods: TSCCA cells in logarithmic growth stage were divided into 伪 catulin-siRNA transfection group, empty plasmid transfection group and blank control group. RT-PCR assay was used to detect the relative expression of 伪 catulin mRNA in TSCCA cells. The relative expression of 伪-catulin protein in TSCCA cells was detected by Western blotting assay, the cell growth inhibition rate was detected by MTT assay, and the apoptosis rate of TSCCA cells in each group was detected by flow cytometry. Results: compared with the blank plasmid transfection group and the blank control group, the relative expression level of 伪-catulin mRNA in the TSCCA cells of the 伪-catulin-siRNA transfection group was decreased (P0.01), and the relative expression of 伪-catulin mRNA was prolonged with time. The relative expression level of 伪-catulin mRNA decreased gradually, and was lower than that at 24 and 48 h at 72 h (P0.01). Western blotting method), compared with the blank plasmid transfection group and the blank control group. The relative expression level of 伪-catulin protein in TSCCA cells was decreased in 伪-catulin-siRNA transfection group (P0.05). MTT method). Compared with empty plasmid transfection group and blank control group, the growth inhibition rate of TSCCA cells in 伪-catulin-siRNA transfection group was higher than that in blank control group (P0. 001). Compared with empty plasmid control group and blank control group, the apoptosis rate of TSCCA cells in 伪-catulin-siRNA transfection group was higher than that in blank control group (P0. 001). Conclusion: specifically silencing the expression of 伪-catulin gene can inhibit the proliferation of TSCC TSCCA cells and promote its apoptosis.
【作者单位】：辽宁医学院附属第一医院口腔科;
【基金】：辽宁省科技厅自然科学基金资助课题(2015020333)
【分类号】：R739.86

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