肌上皮细胞在腮腺再生过程中的变化

发布时间：2018-12-06 21:09

【摘要】：目的研究腮腺再生过程中的肌上皮细胞(MEC)数量及分布的变化。方法 54只Wistar大鼠分为8个实验组和1个正常对照组,每组6只。实验组结扎大鼠右侧腮腺主导管,结扎后第14天使其再通,分别于再通后第0、1、3、5、7、10、14、21天获取腮腺组织标本,应用苏木精-伊红染色观察再生腺体的组织学变化,并采用免疫组织化学染色法定量分析MEC在腮腺再生不同时间点的数量及分布情况,并与正常对照组作比较。结果腮腺组织于主导管结扎后第14天明显萎缩,多数腺泡细胞消失,导管样结构明显增多;而MEC数量明显增加,主要分布在导管样结构周围。导管再通后,从第3天开始,腺泡细胞明显增加,导管样结构明显减少,同时MEC数量减少,主要分布在新生腺泡及导管样结构周围,导管再通第3、5天时MEC数量下降最为明显;再通14 d后,腺体结构和MEC的数量及分布基本恢复正常,与正常对照组无明显差异。结论 MEC的数量和分布在腮腺萎缩后再生的第14天可基本恢复正常,腮腺的再生主要发生在主导管再通后的5 d内。
[Abstract]:Objective to study the quantity and distribution of (MEC) in myoepithelial cells during parotid gland regeneration. Methods 54 Wistar rats were divided into 8 experimental groups and 1 normal control group with 6 rats in each group. The parotid gland tissue specimens were obtained from the right parotid gland of the rats in the experimental group after ligation and recanalization of the 14th angel after ligation. The histological changes of the regenerated glands were observed by hematoxylin and eosin staining. The quantity and distribution of MEC at different time points of parotid regeneration were analyzed by immunohistochemical staining and compared with normal control group. Results on the 14th day after ligation of the main tube, the parotid gland shrank obviously, most of the acinar cells disappeared, and the catheter-like structure increased obviously, while the number of MEC increased obviously, mainly around the catheter-like structure. After recanalization, acinar cells increased significantly and catheter-like structure decreased, and the number of MEC decreased from the 3rd day after recanalization, mainly distributed around the new acinus and catheter-like structure, and the number of MEC decreased most obviously at the 3rd day after catheter recanalization. After 14 days of recanalization, the number and distribution of glandular structure and MEC returned to normal, and there was no significant difference between the two groups. Conclusion the number and distribution of MEC can return to normal on the 14th day after the atrophy of parotid gland, and the regeneration of parotid gland mainly occurs within 5 days after the recanalization of main duct.
【作者单位】：滨州医学院附属医院口腔颌面外科;滨州医学院附属医院口腔正畸科;
【基金】：山东省医药卫生科技发展计划基金资助项目(2011HW004)
【分类号】：R739.8

【参考文献】