Akt抑制剂MK2206对舌鳞癌TCA-8113细胞增殖和凋亡的影响及其作用机制

发布时间：2018-12-07 16:42

【摘要】：目的:探讨Akt抑制剂MK2206对舌鳞状细胞癌(舌鳞癌)TCA-8113细胞增殖和凋亡的影响,并阐明其可能的作用机制。方法:取处于对数生长期舌鳞癌TCA-8113细胞株随机分为对照组和1、5、25、125及250nmol·L-1 MK2206实验组。在不同剂量及时间处理因素下,采用MTT法检测细胞增殖抑制率,流式细胞术(FCM)检测细胞凋亡率,蛋白质印迹分析检测细胞中caspase-9、Bad、GSK-3β、p-Akt和T-Akt蛋白表达水平。结果:舌鳞癌TCA-8113细胞在MK2206作用12、24和36h的半数抑制浓度(IC50)分别为(112.54±1.67)、(79.67±2.01)和(33.33±1.98)nmol·L-1。FCM法检测,1、5、25、125和250nmol·L-1 MK2206作用舌鳞癌TCA-8113细胞12h后,细胞凋亡率分别为(14.2±0.74)%、(19.3±0.45)%、(35.1±0.45)%、(39.6±0.48)%和(52.1±0.19)%,与对照组比较差异均有统计学意义(P0.01)。蛋白质印迹分析,随着MK2206药物剂量和作用时间的增加,p-Akt、Bad和GSK-3β蛋白表达水平下降,与对照组β-actin比较其条带的颜色变暗;caspase-9蛋白表达水平升高,与对照组β-actin比较其条带的颜色变深。T-Akt蛋白表达变化不明显,与对照组β-actin比较条带的颜色无明显不同。结论:Akt抑制剂MK2206可抑制舌鳞癌TCA-8113细胞增殖并诱导凋亡。
[Abstract]:Aim: to investigate the effects of Akt inhibitor MK2206 on the proliferation and apoptosis of TCA-8113 cells in tongue squamous cell carcinoma (TSCC) and to elucidate its possible mechanism. Methods: TCA-8113 cell lines of tongue squamous cell carcinoma at logarithmic growth stage were randomly divided into three groups: control group and 250nmol L-1 MK2206 group. The inhibition rate of cell proliferation was detected by MTT assay, apoptosis rate was detected by flow cytometry (FCM), and caspase-9,Bad,GSK-3 尾 in cells was detected by Western blot analysis. P-Akt and T-Akt protein expression level. Results: the median inhibitory concentrations (IC50) of TCA-8113 cells in tongue squamous cell carcinoma were (112.54 卤1.67), (79.67 卤2.01) and (33.33 卤1.98) nmol L-1.FCM after MK2206 treatment for 12h and 36h, respectively. The apoptosis rates of TCA-8113 cells treated with 25125 and 250nmol L-1 MK2206 for 12 h were (14.2 卤0.74)%, (19.3 卤0.45)%, (35.1 卤0.45)%, (39.6 卤0.48)% and (52.1 卤0.19)%, respectively. Compared with the control group, the difference was statistically significant (P0.01). Western blotting analysis showed that the expression of p-Akttbuad and GSK-3 尾 protein decreased with the increase of MK2206 dosage and action time. Compared with the control group, the band color of p-Aktbud and GSK-3 尾 was darkened. The expression of caspase-9 protein increased, and the color of the band became darker than that of the control group. The expression of T-Akt protein did not change significantly, but the color of the band was not different from that of the control group. Conclusion: Akt inhibitor MK2206 can inhibit the proliferation and induce apoptosis of TCA-8113 cells in tongue squamous cell carcinoma.
【作者单位】：辽宁医学院附属第一医院口腔科;
【基金】：辽宁省教育厅高等学校科学研究项目资助课题(L2010315)
【分类号】：R739.86

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