富自体浓缩生长因子膜在兔下颌骨牵张成骨中的作用

发布时间：2018-12-13 11:19

【摘要】：背景:富自体浓缩生长因子膜可参与调节代谢,已被用于骨缺损的重建。研究发现在牵张成骨的新骨缝附近的成骨细胞、间质细胞及骨细胞的胞质中均有骨保护素和核因子ΚB受体活化因子配体表达。目的:分析骨矫形作用的机制及富自体浓缩生长因子膜对兔下颌骨牵张成骨的促进意义。方法:随机选取24只大白兔建立兔下颌骨牵张成骨模型;对照组行左单侧下颌骨牵张成骨;实验组将富自体浓缩生长因子膜固定于牵张成骨器内侧面并且完全包绕牵张间隙,再行右单侧下颌骨牵张成骨;共延长6 mm。在固定期第1,7,14及28天分别处死动物,获取双侧下颌骨行组织学苏木精-伊红染色、免疫印迹法Western blot法和免疫组织化学检测核因子ΚB受体活化因子配体及骨保护素在新生骨中的表达情况;观察和对比两组间牵张间隙内成骨效果。结果与结论:牵引后第1,7,14天骨保护素表达的阳性细胞率和阳性面积百分比实验组均显著高于对照组(P0.05或P0.01);牵引后第1,14天核因子ΚB受体活化因子配体表达的阳性细胞率和阳性面积百分比实验组均显著高于对照组(P0.05或P0.01);Western blot法检测核因子ΚB受体活化因子配体/骨保护素比值对照组较实验组要高(P0.01)。结果说明,富自体浓缩生长因子膜可促进兔下颌骨牵张成骨间隙内的新骨形成和矿化,说明富自体浓缩生长因子膜是促进下颌骨牵张成骨的有效手段。
[Abstract]:Background: rich-self-enriched growth factor membrane is involved in the regulation of metabolism and has been used in the reconstruction of bone defect. It was found that osteoprotegerin and nuclear factor K B receptor activating factor ligand were expressed in osteoblasts, interstitial cells and osteocytes near the new suture of distraction osteogenesis. Aim: to analyze the mechanism of orthopedic bone formation and the significance of autogenous concentrated growth factor membrane in promoting distraction osteogenesis of rabbit mandible. Methods: 24 rabbits were randomly selected to establish mandibular distraction osteogenesis model, and the left unilateral mandibular distraction osteogenesis was performed in the control group. In the experimental group, the autogenous concentrated growth factor membrane was fixed on the medial side of the distraction osteoblasts and completely wrapped in the distraction space, and then the right unilateral mandibular distraction osteogenesis was performed, which was extended for a total of 6 mm.. The animals were killed on the 14th and 28th day of fixation, and the bilateral mandibles were stained with hematoxylin and eosin. The expression of nuclear factor K B receptor activating factor ligand and osteoprotegerin in newborn bone were detected by Western blot and immunohistochemistry. The effect of osteogenesis in distraction space was observed and compared between the two groups. Results and conclusion: the positive cell rate and percentage of positive area of osteoprotegerin expression in the experimental group were significantly higher than those in the control group (P0.05 or P0.01) on day 714 after traction. On the 14th day after traction, the positive cell rate and the percentage of positive area in the experimental group were significantly higher than those in the control group (P0.05 or P0.01). The ratio of nuclear factor K B receptor activating factor ligand / osteoprotegerin in the control group was higher than that in the experimental group (P0.01). The results showed that the rich autogenous concentrated growth factor membrane could promote the formation and mineralization of new bone in the mandibular distraction osteogenesis space, which indicated that the rich autologous concentrated growth factor membrane was an effective method to promote the mandibular distraction osteogenesis.
【作者单位】：辽宁医学院附属第二医院口腔外科;锦州医科大学发育生物学教研室;
【基金】：辽宁省教育厅基金项目(L2012308)~~
【分类号】：R782

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