纳米纤维素蛋白和脱细胞基质修复口腔黏膜

发布时间：2018-12-14 22:30

【摘要】：背景:口腔黏膜的敏感性和分泌黏液的功能等增加了口腔软组织修复难度,患者修复后其形态和功能难以达到预期的修复效果。纳米纤维素蛋白主要由甘氨酸、丙氨酸以及丝氨酸等组成,该材料具有良好的组织相容性。但是,目前临床上对于纳米纤维素蛋白和脱细胞基质在口腔黏膜修复中效果效果比较研究较少,且临床上对于两种材料修复效果尚存在较大争议。目的:观察纳米纤维素蛋白和脱细胞基质在口腔黏膜修复中的应用,比较两种不同修复材料的效果。方法:建立缺口黏膜缺损模型大鼠,建模后随机分为4组,对照组大鼠采用凡士林油修复,纳米纤维素蛋白组大鼠采用纳米纤维素蛋白修复,牛脱细胞基质组采用牛皮肤组织脱细胞基质修复,人脱细胞基质组则利用人皮肤组织脱细胞基质修复。各组大鼠进行2个月观察,比较不同材料下大鼠口腔黏膜中的修复效果。结果与结论:(1)口腔黏膜缺损直径:采用游标卡尺对4组口腔黏膜修复进行测量,各组大鼠术后1 d口腔黏膜缺损直径差异无显著性意义(P0.05),纳米纤维素蛋白组大鼠术后3,5,7 d口腔黏膜缺损直径显著低于其他3组(P0.05),牛脱细胞基质组和人脱细胞基质组在术后5,7 d口腔黏膜缺损直径显著低于对照组(P0.05);(2)口腔黏膜组织形态:光学显微镜下观察显示,纳米纤维素蛋白组、牛脱细胞基质组和人脱细胞基质组在术后1,3,5,7周口腔黏膜缺损部分新生组织中毛细血管内皮数差异无显著性意义(P0.05);纳米纤维素蛋白组、牛脱细胞基质组和人脱细胞基质组与对照组相比差异有显著性意义(P0.05);纳米纤维素蛋白组术后21 d新生上皮较厚,排列比较紧密;牛脱细胞基质组术后21 d缺损部位修复良好,新生上皮存在,炎性细胞减少;人脱细胞基质组存在明显炎性细胞,存在新生上皮组织,厚度一般;对照组修复21 d后炎性细胞较多,上皮组织较薄。(3)结果说明,纳米纤维素蛋白材料和脱细胞基质能促进口腔黏膜上皮增生,加快创面愈合。
[Abstract]:Background: the sensitivity of oral mucosa and mucus secreting function increase the difficulty of repairing oral soft tissue, and the shape and function of patients are difficult to achieve the desired repair effect. Nanocellulose protein is mainly composed of glycine, alanine and serine, which has good histocompatibility. However, there are few clinical studies on the effects of nano-cellulose protein and acellular matrix in oral mucosal repair. Aim: to observe the application of nano-cellulose protein and acellular matrix in oral mucosal repair and compare the effects of two kinds of repair materials. Methods: the rats with notched mucosal defect were randomly divided into 4 groups. The control rats were repaired with vaseline oil and the rats with nano-cellulose protein were repaired with nano-cellulose protein. Bovine acellular matrix group was repaired by acellular matrix of bovine skin and human acellular matrix group was repaired by human skin acellular matrix. Rats in each group were observed for 2 months to compare the repair effect of oral mucosa in rats with different materials. Results and conclusion: (1) the diameter of oral mucosal defect was measured by Vernier caliper. There was no significant difference in the diameter of oral mucosal defect 1 day after operation in each group (P0.05). The diameter of oral mucosal defect in nano-cellulose protein group was significantly lower than that in the other three groups (P0.05). The diameter of oral mucosal defect in bovine acellular matrix group and human acellular matrix group was significantly lower than that in control group on the 7th day after operation (P0.05). (2) Morphology of oral mucosa: observed under optical microscope, nano-cellulose protein group, bovine acellular matrix group and human acellular matrix group were observed at 1: 3 after operation. There was no significant difference in the number of capillary endothelium in some new tissues of oral mucosal defect at 5 ~ 7 weeks (P0.05). Nanocellulose protein group, bovine acellular matrix group and human acellular matrix group had significant differences compared with the control group (P0.05). The defect site of bovine acellular matrix group was repaired well 21 days after operation, the neonate epithelium existed and the inflammatory cells decreased, while in the human acellular matrix group, there were obvious inflammatory cells, new epithelium and normal thickness. In the control group, there were more inflammatory cells and thinner epithelium after 21 days of repair. (3) the results showed that nano-cellulose protein material and acellular matrix could promote the proliferation of oral mucosal epithelium and accelerate wound healing.
【作者单位】： 西南医科大学口颌面修复重建和再生实验室;西南医科大学附属口腔医院牙周黏膜科;西南医科大学附属口腔医院修复科;
【基金】：四川医科大学口腔医学院省级大学生创新训练计划项目(201510632020);四川医科大学口腔医学院校级大学生创新训练计划项目(2015131);四川医科大学附属口腔医院院级课题;四川医科大学2015年国家级大学生创新创业训练计划项目(201510632020) 西南医科大学附属口腔医院院级课题(201507)~~
【分类号】：R783
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本文编号：2379428