不同形貌纯钛表面对人脐静脉内皮细胞生物学行为的影响

发布时间：2018-12-15 11:43

【摘要】：目的:以人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVECs)为模型细胞,研究纯钛表面的形貌因素对种植体周围成血管效应的可能影响。方法:构建不同结构形貌的纯钛表面,包括光滑表面、纳米结构表面、微米结构表面以及微米/纳米复合结构表面,检测不同形貌纯钛表面对HUVECs的黏附和增殖能力,检测血管内皮生长因子(vascular endothelial growth factor,VEGF)的分泌能力和HUVECs内钙离子(calcium,Ca2+)的浓度。结果:不同形貌纯钛材料在扫描电子显微镜下分别具有光滑表面、纳米结构表面、纳米结构表面和微米/纳米复合结构表面。扫描电子显微镜和细胞骨架染色结果显示,在不同形貌纯钛表面孵育24 h后,光滑表面结构组的细胞数目从(18±4)个/视野增加到(42±6)个/视野,纳米结构表面组的细胞数目从(28±6)个/视野增加到(52±10)个/视野,微米结构表面组的细胞数目从(20±4)个/视野增加到(21±6)个/视野,微米/纳米复合结构组表面的细胞数目从(16±4)个/视野增加到(18±6)个/视野,微米结构表面组和微米/纳米复合结构表面组黏附和增殖的细胞数目显著少于光滑表面结构组和纳米结构表面组(P0.05)。酶联免疫吸附定量研究显示,HUVECs在光滑结构表面、纳米结构表面、微米结构表面以及微米/纳米复合结构表面VEGF分泌量分别为(690±35)ng/L、(560±20)ng/L、(474±43)ng/L、(517±29)ng/L,与光滑结构表面相比,HUVECs在微米结构表面和微米/纳米复合结构表面分泌的VEGF量显著减少(P0.05)。Ca2+免疫荧光探针检测结果显示,HUVECs细胞内Ca2+在微米结构表面和微米/纳米复合结构表面的表达显著高于在光滑结构表面和纳米结构表面。结论:HUVECs在微米结构表面和微米/纳米复合结构表面的功能受影响可能与在这些表面HUVECs内钙浓度的增加相关,因此,种植体表面的形貌因素可能通过影响内皮细胞的功能,对种植体周围的成血管效应造成影响。
[Abstract]:Aim: to study the effect of the surface morphology of pure titanium on the vascularization around the implant using human umbilical vein endothelial cells (human umbilical vein endothelial cell,HUVECs) as model cells. Methods: pure titanium surfaces with different morphologies were constructed, including smooth surfaces, nanostructured surfaces, micron surfaces and micron / nanocomposite surfaces. The adhesion and proliferation of pure titanium surfaces with different morphologies to HUVECs were investigated. The secretory capacity of vascular endothelial growth factor (vascular endothelial growth factor,VEGF) and the concentration of calcium ion (calcium,Ca2) in HUVECs were measured. Results: the pure titanium materials with different morphologies had smooth surfaces, nanostructured surfaces and micron / nanocomposite surfaces respectively under scanning electron microscopy (SEM). The results of scanning electron microscopy and cytoskeleton staining showed that the number of cells in smooth surface structure increased from (18 卤4) / field to (42 卤6) / visual field after incubation on titanium surface with different morphologies for 24 hours. The number of cells in the nanostructure surface group increased from (28 卤6) / field to (52 卤10) / field, and that in the micron structure surface group increased from (20 卤4) / field to (21 卤6) / field. The number of cells on the surface of micron / nanocomposite structure increased from (16 卤4) to (18 卤6) / field. The number of cells adhering and proliferating on the surface of micron structure and micron / nanocomposite structure was significantly less than that of smooth surface group and nanostructure surface group (P0.05). Quantitative analysis of enzyme linked immunosorbent assay (Elisa) showed that the amount of VEGF secreted by HUVECs was (690 卤35) ng/L, (560 卤20) ng/L, (474 卤43) ng/L, on smooth structure surface, nano-structure surface, micron structure surface and micron / nanocomposite structure surface, respectively. The amount of VEGF secreted by (517 卤29) ng/L, on the surface of micron structure and on the surface of micron / nanocomposite structure was significantly lower than that on the smooth surface (P0.05). The results of Ca2 immunofluorescence probe showed that, The expression of Ca2 in HUVECs cells was significantly higher on the surface of micron structure and micron / nanocomposite structure than on the smooth surface and nanostructure surface. Conclusion: the function of HUVECs on the surface of micron structure and on the surface of micron / nanocomposite structure may be related to the increase of calcium concentration in HUVECs on these surfaces. Therefore, the morphology of implant surface may affect the function of endothelial cells. Effects on vascularization around implants.
【作者单位】：北京大学口腔医学院·口腔医院修复科;北京大学口腔医学院·口腔医院特诊科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室;
【基金】：国家自然科学基金(81171000,81400563)资助~~
【分类号】：R783.6

【相似文献】