纳米银对变异链球菌标准株及耐氟株抗菌性的研究

发布时间：2018-12-16 20:19

【摘要】：目的对比葡萄糖和次磷酸钠分别还原硝酸银制备两种纳米银的性能,检测两种纳米银对变异链球菌标准株及耐氟株的抑菌圈直径、最小抑菌浓度(MIC)、最小杀菌浓度(MBC),在两种纳米银对实验菌的抗菌效果方面进行初步研究。方法采用葡萄糖和次磷酸钠分别还原硝酸银制备两种纳米银,通过UV-Vis、XRD及TEM三种方法对两种纳米银进行表征；利用变异链球菌标准株以及前期实验室成功构建并保存的变异链球菌耐氟株为实验菌株,制备两实验菌株的TPY平板,分别放上两种纳米银溶胶滤纸片,将平板倒置、放于37℃恒温箱微需氧过夜培养16h(以下培养条件相同),观察并测量抑菌圈直径；取两块96孔板,在每一板内分别向第一行的1到10孔内依次加入100μL二倍稀释后的银溶胶,然后吸取100μL稀释为106CFU/mL的两菌液分别加到两板的以上各孔内,密封孔板,观察各孔的浑浊度(同理检测另一种纳米银)；从未浑浊的实验孔里分别取100μL液体,滴到TPY固体培养基上,涂布均匀,观察并计数菌板上实验菌生长情况。测定两种纳米银对实验菌株的抑菌圈直径、最小抑菌浓度(MIC)、最小杀菌浓度(MBC)。 结果葡萄糖和次磷酸钠分别制备的纳米银均为：分散性好、稳定性较好、均粒径分别约为13.40nm、15.50nm的球形粒子,且前者的性能优于后者；葡萄糖制备的纳米银对标准株及耐氟株的抑菌圈直径分别为(20.87±0.66) mm、(19.68±0.86)mm,MIC分别为(2.08±0.90)μg/mL、(2.60±0.90)μg/mL, MBC分别为(4.16±1.81)μg/mL、(5.21±1.81)μg/mL、；次磷酸钠制备的纳米银对标准株及耐氟株的抑菌圈直径分别为(14.86±O.43)mm.(14.19±0.50)mm, MIC为(35.15±12.18)μg/mL、(56.24±24.36)μg/mL,MBC为(70.30±24.36) μg/mL、(1l2.48±48.72)μg/mL。 结论葡萄糖和次磷酸钠分别还原硝酸银制备了分散性好、稳定性较好、均粒径较小约为13.40nm、15.50nm球形粒子的纳米银；两种纳米银对变异链球菌标准株及耐氟株均有较高的抗菌性,且前者的抗菌性较后者好,但同种纳米银对标准株和耐氟株抗菌性的差异不显著。
[Abstract]:Objective to compare the properties of glucose and sodium hypophosphate in reducing silver nitrate to prepare two kinds of silver nanoparticles, and to detect the diameter of bacteriostasis circle, minimum inhibitory concentration (MIC),) and minimal bactericidal concentration (MBC),) of two kinds of silver nanoparticles to Streptococcus mutans standard strain and fluorine-resistant strain. The antimicrobial effects of two kinds of silver nanoparticles against experimental bacteria were preliminarily studied. Methods two kinds of silver nanoparticles were prepared by reducing silver nitrate by glucose and sodium hypophosphite respectively. The two kinds of silver nanoparticles were characterized by UV-Vis,XRD and TEM. Using the standard strain of Streptococcus mutans and the fluorine-resistant strain of Streptococcus mutans successfully constructed and preserved in the laboratory, the TPY plates of the two strains were prepared. The microaerobic overnight culture was carried out in 37 鈩，

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