乙醇润湿对根部牙本质表面形貌及纳米粘附力的影响

发布时间：2018-12-29 07:36

【摘要】：目的利用原子力显微镜观察乙醇润湿处理后牙本质的表面形貌,并检测其表面纳米粘附力的改变,探讨乙醇润湿技术用于提高牙本质粘接效果的临床作用机制。方法将人单根管前磨牙制成根部牙本质片并打磨抛光、酸蚀冲洗后,随机分成5组,分别用无水乙醇处理0s(对照组)、20s、60s、3×60s或梯度乙醇处理样本;原子力显微镜在空气环境中检测乙醇润湿后的牙本质表面特性;利用SPSS 16.0对结果进行单因素方差分析和Tukey检验。结果和对照组相比,随着乙醇使用时间的延长,牙本质表面波浪形的形貌越不明显,逐渐趋于平滑,牙本质纳米粘附力明显降低(P0.001),其中3×60s组和梯度乙醇组的值最低,且两组间差异无统计学意义(P0.05)。结论在空气环境中采用原子力显微镜检测时,随着乙醇使用时间的延长,牙本质表面波浪形的形貌逐渐趋于平滑,纳米粘附力越低,表明增加乙醇润湿处理时间可使牙本质胶原纤维网中的水分置换越充分,提高牙本质粘接界面的疏水性。
[Abstract]:Objective to observe the surface morphology of dentin after ethanol wetting by atomic force microscope (AFM), and to investigate the mechanism of ethanol wetting in improving the adhesion effect of dentin. Methods the human premolars were made into root slices and polished. After acid etching, they were randomly divided into 5 groups. The samples were treated with anhydrous ethanol for 0s (control group), 20 s for 60 s or 3 脳 60 s for gradient ethanol. Atomic force microscopy (AFM) was used to detect the dentin surface characteristics after ethanol wetting, and SPSS 16.0 was used to test the results by univariate ANOVA and Tukey test. Results compared with the control group, with the prolongation of ethanol use time, the shape of wave shape of dentin surface became less obvious, and gradually tended to smooth, and the adhesion force of dentine nanoparticles decreased significantly (P0.001). The values of 3 脳 60s group and gradient ethanol group were the lowest. There was no significant difference between the two groups (P0.05). Conclusion when measured by atomic force microscope in air environment, the wave morphology of dentin surface tends to smooth with the prolongation of ethanol use time, and the lower the adhesion force of nanometer is. The results showed that increasing the time of ethanol wetting could improve the hydrophobicity of dentin adhesion interface by increasing the water displacement in the dentin collagen fiber network.
【作者单位】：陕西省颅颌面精准医学研究重点实验室;西安交通大学口腔医院修复科;武汉大学口腔医院修复科;武汉大学口腔医院颌面外科;
【基金】：国家自然科学基金资助项目(No.81400551)~~
【分类号】：R783.1

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