脂多糖对大鼠牙髓细胞ALP、BSP、DSPP表达的影响

发布时间：2019-01-01 11:11

【摘要】：目的:研究脂多糖(lipopolysaccharide,LPS)对大鼠牙髓细胞牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)、骨涎蛋白(bone sialoprotein,BSP)及碱性磷酸酶(alkaline phosphatase,ALP)表达的影响。方法:采用组织块法获得大鼠牙髓细胞,体外常规培养并进行鉴定,以含0.1、1、10、100和10000 ng/mL牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)的LPS作用牙髓细胞1、3、5 d,用实时定量PCR检测DSPP、ALP、BSP mRNA表达的变化,采用SPSS17.0软件包对数据进行统计学分析。结果:镜下贴壁后的细胞形态多样,多呈成纤维样细胞形态,还有部分多角形细胞,胞质突起。实时定量PCR结果显示,与对照组相比,1、10 ng/mL LPS组大鼠牙髓细胞DSPP、ALP、BSP的mRNA表达增高,100、10000 ng/mL LPS组DSPP、ALP、BSP的mRNA表达均降低;在1、3、5 d时,1、10、100和10000 ng/mL LPS组mRNA表达逐渐减少。0.1 ng/mL LPS对mRNA的表达无显著影响。3种因子呈现相似的表达变化趋势。结论:低剂量P.g LPS能促进牙髓细胞ALP、BSP、DSPP的表达,高剂量时则抑制ALP、BSP、DSPP的表达;随着培养时间的延长,促进作用逐渐减弱,抑制作用逐渐增强。
[Abstract]:Aim: to study the effects of lipopolysaccharide (lipopolysaccharide,LPS) on the expression of sialoprotein (dentin sialophosphoprotein,DSPP), bone sialoprotein (bone sialoprotein,BSP) and alkaline phosphatase (alkaline phosphatase,ALP) in rat dental pulp cells. Methods: rat dental pulp cells were obtained by tissue mass method. The pulp cells were cultured and identified in vitro. The pulp cells were treated with LPS containing 0.1 ng/mL porphyromonas gingivalis (Porphyromonas gingivalis,P.g) for 5 days. Real-time quantitative PCR was used to detect the expression of DSPP,ALP,BSP mRNA and SPSS17.0 software package was used to analyze the data. Results: the morphologies of the cells attached to the wall under microscope were various, mostly fibroblast-like cells, and some polygonal cells with cytoplasmic processes. The results of real-time quantitative PCR showed that compared with the control group, the mRNA expression of DSPP,ALP,BSP in dental pulp cells was increased in 1T 10 ng/mL LPS group, and the mRNA expression of DSPP,ALP,BSP was decreased in 10010 000 ng/mL LPS group. After 5 days, the expression of mRNA was gradually decreased in 110100 and 10000 ng/mL LPS groups. 0. 1 ng/mL LPS had no significant effect on the expression of mRNA. The three factors showed a similar trend of expression. Conclusion: low dose P.g LPS can promote the expression of ALP,BSP,DSPP in dental pulp cells and inhibit the expression of ALP,BSP,DSPP at high dose.
【作者单位】： 青岛大学医学院;青岛大学附属医院口腔内科;
【基金】：青岛市科技支撑计划项目(10-3-3-3-6-nsh)~~
【分类号】：R781

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