化湿行瘀清热方剂对口腔扁平苔藓患者唾液中差异蛋白的影响

发布时间：2019-01-05 10:30

【摘要】：目的:筛选鉴定口腔扁平苔藓(OLP)治疗前后唾液中的差异蛋白,探讨化湿行瘀清热方剂对口腔扁平苔藓患者唾液中差异蛋白的影响。方法:收集OLP患者化湿行瘀清热方剂治疗前后的唾液,提取唾液总蛋白,应用双向荧光差异凝胶电泳和液相色谱-质谱联用技术筛选鉴定差异蛋白,蛋白质印迹法检测差异蛋白的表达情况。结果:筛选鉴定出OLP患者化湿行瘀清热方剂治疗前后6种差异蛋白:分泌型IgA、血清白蛋白、IgM、唾液淀粉酶、碳酸酐酶6、锌α2糖蛋白。OLP患者治疗后较治疗前唾液中IgA表达量升高,血清白蛋白、IgM、碳酸酐酶6和锌α2糖蛋白治疗后较治疗前表达量下降,差异具有统计学意义(P0.05)。结论 :OLP患者化湿行瘀清热方剂治疗前后唾液中存在差异蛋白,并可能在OLP的发生发展中起着重要作用。
[Abstract]:Objective: to screen and identify saliva differential proteins in oral lichen planus (OLP) before and after (OLP) treatment, and to explore the effect of Huashingxingyu Qingre prescription on saliva differential protein in patients with oral lichen planus (OLP). Methods: saliva was collected from patients with OLP before and after treatment with Huashingxingyu Qingre prescription, and saliva total protein was extracted. Two-dimensional fluorescence differential gel electrophoresis and liquid chromatography-mass spectrometry were used to screen and identify differentially expressed proteins. The expression of differentially expressed proteins was detected by Western blot. Results: six kinds of differential proteins of OLP patients before and after treatment were identified: secretory IgA, serum albumin, IgM, saliva amylase, carbonic anhydrase, carbonic anhydrase. Serum albumin, IgM, carbonic anhydrase 6 and zinc 伪 2 glycoprotein decreased after treatment in patients with OLP (P0.05). Conclusion: there are different proteins in saliva of patients with OLP before and after treatment, and may play an important role in the occurrence and development of OLP.
【作者单位】：河北医科大学第四医院口腔科;
【基金】：国家自然基金课题(编号:81273813) 河北省科技厅(编号:12276104D-41) 河北省中医药管理局(编号:2011013) 河北省卫生厅(编号:20100125)
【分类号】：R276.8

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