人颊黏膜微生物群落年龄相关性演替研究

发布时间：2019-01-07 18:22

【摘要】：目的初步探讨健康人群颊黏膜菌群组成在不同年龄及牙列阶段的动态演替过程。方法 25例健康受试者根据年龄及牙列情况分为5组:乳牙列组、混合牙列组、青少年组、青年组及老年组;分别提取各样本的细菌总DNA进行聚合酶链式反应,采用Quantity One软件对扩增产物的变性梯度凝胶电泳(DGGE)指纹图谱进行生物信息学分析。结果 1)颊黏膜菌群的组成具有个体差异性。2)乳牙列组、混合牙列组、青少年组、青年组及老年组DGGE指纹图谱的平均条带数分别为21.2±4.0、17.8±3.9、15.8±4.3、16.8±3.7、22.2±6.5,各组间的差异无统计学意义(P0.05),提示颊黏膜优势菌群的数量在各个年龄阶段保持相对稳定。3)5组的Shannon指数分别为1.73±0.2、1.43±0.1、1.05±0.2、1.45±0.2和1.63±0.3,各组间的差异有统计学意义(P=0.003);提示颊黏膜微生物的多样性在儿童至青少年阶段呈下降趋势,在青年至老年阶段呈上升趋势。4)群落结构相似性聚类分析发现:同组内大部分样本聚类位置相近,群落结构表现出较高的相似性;不同组的大部分样本未聚类在一起,群落结构呈现出一定的差异性。结论不同年龄组健康人群的颊黏膜微生物群落组成存在差异,青少年期可能是颊黏膜微生物多样性改变的重要转折点。
[Abstract]:Objective to investigate the dynamic succession process of buccal mucosal flora in healthy population at different ages and dentition stages. Methods 25 healthy subjects were divided into 5 groups according to their age and dentition: primary dentition group, mixed dentition group, adolescent group, youth group and elderly group. The total bacterial DNA from each sample was extracted for polymerase chain reaction, and the denaturing gradient gel electrophoresis (DGGE) fingerprint of the amplified product was analyzed by Quantity One software for bioinformatics analysis. Results 1) the composition of buccal mucosal flora had individual difference. 2) the primary dentition group, mixed dentition group, adolescent group, and so on. The average number of DGGE fingerprints in the young group and the old group was 21.2 卤4.0 卤17.8 卤3.9U, 15.8 卤3.316.8 卤3.31,22.2 卤6.5, respectively. There was no significant difference among the three groups (P0.05). The results indicated that the number of dominant bacteria in buccal mucosa remained relatively stable at all ages. 3) the Shannon index of the 5 groups was 1.73 卤0.2U 1.43 卤0.1g 1.05 卤0.2g 1.45 卤0.2,1.63 卤0.3, respectively. There was significant difference among the three groups (P0. 003). The results showed that the diversity of buccal mucosal microorganism decreased in childhood to adolescence, and increased in youth and old age. 4) the cluster analysis of community structure similarity showed that most of the samples in the same group were similar in cluster location. The community structure showed high similarity. Most samples of different groups were not clustered together, and the community structure showed some differences. Conclusion there are differences in the composition of buccal mucosal microbial community among healthy people in different age groups, and the youth may be an important turning point for the change of buccal mucosal microbial diversity.
【作者单位】：口腔疾病研究国家重点实验室华西口腔医院(四川大学);
【基金】：国家科技部“973计划”前期研究专项课题基金资助项目(2011CB512108) 国家科技部“十二五”支撑计划基金资助项目(2012BAI07B03) 国家国际科技合作专项基金资助项目(2011-DFA30940)
【分类号】：R780.2

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