根尖周损伤后根管内组织的变化过程及基质细胞衍生因子1释放的实验研究

发布时间：2019-01-09 06:03

【摘要】：研究目的:通过建立SD大鼠上颌第一磨牙无髓根管模型并刺激损伤根尖周组织出血,观察在刺激损伤根尖周后根管内的组织变化特点、基质细胞衍生因子1(stromal cell derived factor-1,SDF-1)在根管内表达情况及损伤前后血清中SDF-1浓度变化。探讨牙髓血管再生中SDF-1的作用。研究方法:6-8周龄SPF级别SD雄性大鼠64只,体重180-220g。建立左侧上颌第一磨牙无髓根管模型后对实验动物进行根尖周刺激损伤,诱导根尖出血(56只)。对损伤组刺激近中根根尖损伤出血并按照根尖周损伤后不同的时间点随机分为损伤12h、1d、2d、4d、7d、10d、14d共7组,麻醉后处死动物并采集血液及上颌骨,酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中SDF-1含量。上颌骨固定、脱钙后行组织化学染色法观察在根尖周损伤后根管内组织的变化情况及SDF-1在根管内组织的表达特点。研究结果:进入根管的根尖血液发生凝血,在损伤后1d、2d及4d,根管内组织表现形式为纤维蛋白包裹血凝块及血细胞。组织损伤后期,根管内血凝块溶解及炎细胞减少,组织之间及组织与牙本质之间连接紧密,损伤后14天组织中出现新生血管结构,组织与根管壁之间出现细胞结构。免疫组织化学染色显示,创伤后根管内组织的血凝块及炎性细胞表达SDF-1,随着时间增加,血凝块及炎性细胞减少,SDF-1表达减弱。ELISA检测显示,根尖周损伤后,12h组及4 d组血清中SDF-1浓度升高(P0.05),差异有统计学意义,其余时间段结果与损伤前相比无显著性变化。研究结论:1.根尖诱导出血后,根管内牙髓血管再生的过程及损伤后SDF-1在根管内组织的表达与血凝块和炎细胞状态相关。2.根尖周损伤后12h及4d,血清中SDF-1浓度升高,但在整个牙髓血管再生过程未能持续,通过刺激根尖周损伤趋化骨髓内干细胞通过血循环归巢的证据需进一步实验才能证实。
[Abstract]:Objective: to observe the changes of tissue and stromal cell derived factor 1 (stromal cell derived factor-1,) in the root canal of maxillary first molar induced by SD by stimulating periapical hemorrhage. SDF-1) expression in root canal and the change of serum SDF-1 concentration before and after injury. To investigate the role of SDF-1 in dental pulp vascular regeneration. Methods: 64 SPF grade SD male rats of 6-8 weeks old, weight 180-220 g. The left maxillary first molar model of unmyelinated root canal was established and the periapical injury was carried out in the experimental animals (56 rats). The injury group was randomly divided into 7 groups according to different time points after periapical injury. The animals were killed and blood and maxillary bone were collected after anesthesia. Enzyme linked immunosorbent assay (enzyme linked immunosorbent assay,ELISA) was used to detect the content of SDF-1 in serum. The changes of root canal tissue and the expression of SDF-1 in root canal were observed by histochemical staining after maxillary fixation and decalcification. The results showed that the blood clotting in the root tip of the root canal showed fibrin in the blood clots and blood cells in the root canal at 2 and 4 days after the injury. In the late stage of tissue injury, hemagglutination and inflammatory cells in the root canal decreased, and the connection between tissue and dentin was close. On the 14th day after injury, neovascularization appeared in the tissue and cell structure appeared between the tissue and the root canal wall. Immunohistochemical staining showed that the expression of SDF-1, in blood clots and inflammatory cells in root canal after trauma increased with time, blood clots and inflammatory cells decreased, and SDF-1 expression decreased. ELISA detection showed that after periapical injury, the expression of SDF-1, was decreased. The serum SDF-1 concentration in 12h group and 4-day group was significantly higher than that before injury (P0.05). Conclusion: 1. The process of pulp vascular regeneration in root canal and the expression of SDF-1 in root canal were related to blood clots and inflammatory cells after apical hemorrhage. 2. The concentration of SDF-1 in serum increased at 12 h and 4 d after periapical injury, but it could not be sustained in the whole process of pulp vascular regeneration. The evidence of blood circulation homing by stimulating periapical injury to chemotaxis of bone marrow stem cells could not be confirmed by further experiments.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781

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