人牙髓细胞条件培养液对人牙囊细胞成骨分化作用的体外研究
发布时间：2019-01-28 11:10
【摘要】：目的:探索人牙髓细胞条件培养液(HDPSCs-CM)对人牙囊细胞(HDFSCs)向成骨分化的作用。方法:利用胶原酶消化法获得HDFSCs,经纯化鉴定后培养于HDPSCs-CM中;CCK-8检测HDFSCs增殖活性,观察细胞形态改变;碱性磷酸酶(ALP)染色、茜素红S染色定性分析细胞成骨能力的改变。qRT-PCR检测HDFSCs中骨膜蛋白(POSTN)、Ⅰ型胶原(Col-Ⅰ)、碱性磷酸酶(ALP)、骨涎蛋白(BSP)以及骨桥蛋白(OPN)的mRNA表达情况。结果:经HDPSCs-CM诱导后,HDFSCs形态发生成牙骨质或成骨样改变;诱导组HDFSCs增殖活性受到明显抑制(P0.05或P0.01);诱导组ALP染色强于对照组,茜素红S染色矿化结节多于对照组;诱导组POSTN、Col-Ⅰ、ALP、BSP、OPN的表达量明显增高(P0.05或P0.01)。结论:HDPSCs-CM能促进HDFSCs成骨分化。
[Abstract]:Aim: to investigate the effect of human dental pulp conditioned medium (HDPSCs-CM) on the osteogenic differentiation of human dental follicle cells (HDFSCs). Methods: HDFSCs, was purified and identified by collagenase digestion and cultured in HDPSCs-CM. The proliferative activity of HDFSCs was detected by CCK-8 and the changes of cell morphology were observed. Alkaline phosphatase (ALP) staining and alizarin red S staining were used to qualitatively analyze the changes of osteogenesis. QRT-PCR detection of (POSTN), 鈪，
本文编号：2417000
[Abstract]:Aim: to investigate the effect of human dental pulp conditioned medium (HDPSCs-CM) on the osteogenic differentiation of human dental follicle cells (HDFSCs). Methods: HDFSCs, was purified and identified by collagenase digestion and cultured in HDPSCs-CM. The proliferative activity of HDFSCs was detected by CCK-8 and the changes of cell morphology were observed. Alkaline phosphatase (ALP) staining and alizarin red S staining were used to qualitatively analyze the changes of osteogenesis. QRT-PCR detection of (POSTN), 鈪，
本文编号：2417000
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