对氧磷酶1 Leu55Met基因多态性与牙周炎伴糖尿病的相关性研究

发布时间：2019-02-13 13:13

【摘要】：目的:研究对氧磷酶(paraoxonase,PON)1 Leu55Met的基因多态性与单纯牙周炎及牙周炎伴糖尿病(diabetes mellitus,DM)(本文特指2型糖尿病)的易感性是否相关。方法:收集慢性牙周炎(chronic periodontitis,CP)伴DM患者、单纯CP患者、单纯DM患者及正常人各50例口腔颊黏膜拭子,采用口腔/咽拭子基因组DNA提取试剂盒提取DNA,然后应用聚合酶链式反应-限制性片段长度多态性检测PON1 Leu55Met的基因型分布。结果:DM伴CP组(CP+DM)、单纯CP组(CP)、单纯DM患组(DM)、正常组(H)在PON1 Leu55Met基因型分布有统计学差异(χ2=12.444,P=0.006)。结论:CP与DM的易感性可能与PON1 Leu55Met基因多态性有关。
[Abstract]:Objective: to investigate whether the gene polymorphism of paraoxonase (paraoxonase,PON) 1 Leu55Met is associated with the susceptibility of periodontitis and periodontitis with diabetes mellitus (diabetes mellitus,DM). Methods: 50 patients with chronic periodontitis (chronic periodontitis,CP) with DM, 50 patients with CP, 50 patients with DM and 50 normal controls were collected to extract DNA, by oral / pharyngeal swab genomic DNA extraction kit. Then polymerase chain reaction-restriction fragment length polymorphism (RFLP) was used to detect the genotype distribution of PON1 Leu55Met. Results: there was statistical difference in PON1 Leu55Met genotype distribution of (H) between DM and CP group (CP DM), simple CP group (CP), simple DM group (DM), normal group (蠂 2 + 12.444U P0. 006). Conclusion: the susceptibility of CP and DM may be related to the polymorphism of PON1 Leu55Met gene.
【作者单位】：重庆医科大学附属口腔医院牙周科口腔疾病与生物医学重庆市重点实验室;
【基金】：重庆市卫生局中医药科研计划资助项目(编号:2010-2-67) 渝中区科学技术委员会资助项目(编号:20110312)
【分类号】：R781.4;R587.1

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