复合纳米银树脂加强型玻璃离子粘结剂银离子释放及体外防脱矿能力的研究

发布时间：2019-02-26 19:27

【摘要】：目的:在树脂加强型玻璃离子(RMGI)粘结剂中添加不同含量(质量比0%,2%,5%,10%)纳米银(NAg)抗菌剂,测量并观察复合粘结剂银离子的释放浓度以及其体外防脱矿能力。方法:1测量不同质量比复合粘结剂银离子的释放浓度:将不同含量NAg/RMGI(质量比0%,2%,5%,10%)混合并充分研磨,按照厂商推荐的方法调拌,制成直径为4mm,厚度为2mm的形状,用光固化灯照射20s使其固化,制成NAg/RMGI试验样本。按照添加的NAg质量比不同分成四组,每组18个,共计72个样本。加入10ml去离子水中浸泡,分别于1天、3天、7天、14天、35天、42天时,利用原子吸收分光度法测量各组复合粘结剂在去离子水中的银离子释放情况。对实验结果进行统计学处理与分析。2镜下观察不同质量比复合粘结剂的防脱矿能力:将75颗人离体双尖牙随机分为五组,以不同NAg/RMGI质量比调和粘接金属托槽(杭州新亚),每组15颗。五组分别为:1组:对照组,用京津釉质粘合剂粘接托槽;2组:树脂加强型玻璃离子粘接托槽;3组:添加2%纳米银树脂加强型玻璃离子粘接托槽;4组:添加5%纳米银树脂加强型玻璃离子粘接托槽;5组:添加10%纳米银树脂加强型玻璃离子粘接托槽。京津釉质粘合剂组:用气枪冲洗吹干牙面,35%磷酸酸蚀60秒,用大量的清水冲洗吹干牙面,牙面呈白垩色。涂封闭剂,用京津釉质粘结剂粘接托槽,定位后去除托槽周围多余的粘结剂;树脂加强型玻璃离子粘合剂组:用气枪冲洗吹干牙面,35%磷酸酸蚀60秒,再次清水冲洗牙面,用润湿的小棉球沾干。按产品说明书推荐的粉液比调和树脂加强型玻璃离子粘结剂粘接托槽,定位后去除托槽周围多余的粘结剂;纳米银树脂加强型玻璃离子组:分别按2%、5%、10%的比例混合NAg及RMGI粘结剂,充分研磨,按产品说明书粘接托槽,定位后用探针将托槽周围多余的粘结剂去除。所有离体牙按组别做好标记,粘结后30分钟,用指甲油在托槽四围lmm外釉质表面开窗,指甲油完全干燥后开始体外PH循环,在人工脱矿液(PH值4.5)中脱矿1.5小时后取出,去离子水冲洗干净,然后将各组牙分别放入装有30m1人工唾液(PH值7.0)的密闭塑料容器中37℃恒温下保存。每天循环两次,共循环30天。去除托槽和牙表面的粘结剂后,在扫描电镜下观察托槽粘结底板及周围lmm区域的釉质表面的形态。结果:1粘结剂银离子的释放:树脂加强型玻璃离子组自始至终没有测到银离子的存在。第1天时2%NAg,5%NAg和10%NAg之间的银离子释放量没有明显的区别(P0.05),3~7天时银离子释放出现快速的增长,尤其以第7天最多,并且随着加入NAg含量的增加,银离子的释放浓度也相应增加,与2%NAg,5%NAg相比较,10%NAg释放了大量的银离子。释放速度随时间的推移而减缓,从第14天以后抗菌粘结剂中银离子释放量基本持平,且各组材料银释放的方式是类似的初期的大量释放和较长时间的低水平持续释放。2 SEM表面观:对照组京津釉质粘结剂组牙釉质表面可见釉间质溶解的多孔性组织改变,呈蜂窝状凹陷;树脂加强型玻璃离子和2%NAg组间牙釉质表面较平整,可见浅表样凹坑,有少许沉积物,两组间没有明显区别。5%NAg,10%NAg组牙釉质表面光滑平整呈鱼鳞样外观,可见沉积样外观,两组间没有明显区别。结论:1银离子可以从复合纳米银树脂加强型玻璃离子中释放到周围环境中,释放量与加入抗菌剂的量成正相关,释放速度随时间的推移而减缓。2树脂加强型玻璃离子中加入一定量的纳米银抗菌剂,在人工脱矿环境中抑制牙釉质脱矿作用增强。
[Abstract]:Objective: To add nano-silver (NAg) antibacterial agent with different content (0%, 2%, 5%, 10%) in the resin reinforced glass ion (RMGI) adhesive, and to measure and observe the release concentration of silver ion and its anti-demineralization ability in vitro. The method comprises the following steps of: 1, measuring the release concentration of the silver ions of the composite bonding agent with different mass ratio: mixing the different content of NAg/ RMGI (mass ratio of 0%, 2%, 5%, 10%) and grinding, mixing according to the method recommended by the manufacturer, and making into a shape with a diameter of 4 mm and a thickness of 2 mm, The photo-curing lamp was used to irradiate the 20s to cure it to prepare the NAg/ RMGI test sample. A total of 72 samples were divided into four groups, 18 in each group, according to the added NAg mass ratio. adding 10ml of deionized water, soaking in deionized water for 1 day, 3 days, 7 days, 14 days, 35 days and 42 days respectively, and using the atomic absorption spectrophotometry to measure the silver ion release condition of each group of composite adhesive in the deionized water. The results of the experiment were statistically processed and analyzed. The anti-demineralization ability of the composite adhesive with different mass ratio was observed under the mirror. The 75 people were randomly divided into five groups from the double-pointed teeth of the body, and the metal supporting groove (Hangzhou Xinya) was mixed with different NAg/ RMGI mass ratio, and 15 in each group. The five groups were: 1 group: control group, adhesion support groove with Beijing-Tianjin enamel adhesive; group 2: resin reinforced glass ionomer cement tray; group 3: addition of 2% nano-silver resin reinforced glass ionomer cement tray; group 4: addition of 5% nano-silver resin reinforced glass ionomer cement tray; 5 groups: 10% nano-silver resin reinforced glass ionomer cement tray was added. Beijing-Tianjin enamel adhesive group: the tooth surface is dried by using air gun, and the surface of the tooth surface is dried by a large amount of clean water for 60 seconds, and the tooth surface is in the chalk color. coating the sealing agent, bonding the tray with the Beijing-Tianjin enamel adhesive, and removing the excess adhesive around the tray after being positioned; and a resin reinforced glass ion adhesive group, which is used for washing and drying the tooth surface with an air gun, the 35 percent of the phosphoric acid is etched for 60 seconds, the tooth surface is washed with clear water, and the wet cotton ball is dipped with the wet cotton ball. and the nano-silver resin reinforced glass ion group is mixed with the NAg and the RMGI adhesive in a proportion of 2 percent, 5 percent and 10 percent, respectively, and is fully ground, The tray shall be bonded according to the product specification, and the excess adhesive around the tray shall be removed with the probe after positioning. All the off-body teeth are marked according to the group, 30 minutes after the bonding, the enamel surface is opened with nail polish on the periphery of the support groove, the nail polish is completely dried, the in-vitro PH circulation is started, the ore is removed for 1 to 5 hours in the artificial demineralization liquid (PHvalue 4.5), and the deionized water is washed clean, The groups of the teeth were then stored at a constant temperature of 37.degree. C.in a closed plastic container with 30ml of artificial saliva (pH 7.0), respectively. Cycle twice a day for 30 days. After removal of the adhesive on the tray and the surface of the teeth, the morphology of the surface of the enamel was observed under the scanning electron microscope. Results: 1 The release of silver ion of the binder: the presence of silver ion was not measured from the beginning to the end of the resin reinforced glass ion group. The release of silver ion between 2% NAg, 5% NAg and 10% NAg at day 1 was not significantly different (P0.05). The release of silver ions at 3-7 days showed a rapid increase, especially at the 7th day, and the release concentration of silver ions increased correspondingly with the increase of the content of the addition of NAg, compared with 2% NAg, 5% NAg. 10% of the nag released a large amount of silver ions. the release rate is slowed over time, and the amount of silver ion release in the antibacterial binder is substantially flat after day 14, and the manner of silver release in each group is a similar initial mass release and a low level of sustained release for a long time. In the control group, the enamel surface of the enamel adhesive group of the Beijing-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin-Tianjin The enamel surface of the 10% NAg group was smooth and flat, and the appearance of the fish scale was observed. There was no obvious difference between the two groups. conclusion: 1 silver ion can be released into the surrounding environment from the reinforced glass ion of the composite nano-silver resin, the release amount is positively correlated with the amount of the addition of the antibacterial agent, the release rate is reduced over time, and a certain amount of nano-silver antibacterial agent is added to the resin-reinforced glass ion, and the effect of demineralization of the enamel is inhibited in the artificial demineralization environment.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783.5

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