骨皮质切开术对Beagle犬前磨牙压入移动影响的三维形态学研究
发布时间：2019-03-02 07:56
【摘要】：组牙的压入移动是正畸治疗的难点之一。正畸临床中常见由于对合牙早失引起的磨牙过度萌出、骨性开合等错合畸形，其矫治均需对后牙进行组牙的压入移动。以往缺乏稳定的支抗是造成组牙压入困难的主要原因，微螺钉种植体支抗（Mini-implant anchorage MIA）的出现为组牙的持续压入提供了良好的解决方案。然而，有研究报道应用种植体支抗压低磨牙会导致根尖以及根分叉区域的牙根吸收。 骨皮质切开术通过对牙齿周围的皮质骨进行牙槽外科手术松解以达到加速牙移动的目的，具有创伤小、操作简单等特点。有临床报道应用骨皮质切开术辅助微种植体支抗进行磨牙压低可以有效加快牙齿移动的速率。目前关于骨皮质切开加速正畸牙移动的动物实验研究主要集中在前牙的内收和矢状向移动，而对于骨皮质切开辅助组牙压入在牙根吸收以及牙槽骨改建方面的三维形态学研究尚未见报道。 因此，有必要建立骨皮质切开辅助组牙压入移动的动物实验模型，对比研究单纯使用微种植体支抗和骨皮质切开结合微种植体支抗辅助组牙压入对于牙齿压低速率，牙根吸收以及牙槽骨改建的影响。 目的：建立骨皮质切开辅助组牙压入的Beagle犬实验动物模型，采用自身对照设计对比研究骨皮质切开对于组牙压入效应的三维形态学影响，同时通过纵向比较不同时间节点的三维形态学变化，探讨骨皮质切开对压入移动的牙齿牙根吸收及牙槽骨改建的影响。 方法：1.建立骨皮质切开辅助组牙压入的动物实验模型。成年雄性Beagle犬8只，随机分为4组，每组2只。随机分配每只Beagle犬的下颌左右侧为实验侧及对照侧，实验侧采用骨皮质切开辅助，微种植体作为支抗对P3，P4进行压低，对照侧则直接应用微种植体支抗压低P3，P4，四个组分别在加力后2周，4周，8周，12周处死，获取标本。 2.牙齿移动速率的大体测量。植入不加力的微种植体作为标记，，对Beagle犬加力后2周、4周、6周、8周、10周、12周牙齿压低量进行大体测量，对比分析实验侧及对照侧的压入移动量；绘制压入量时间曲线，对比分析实验侧及对照侧的牙齿移动速率。 3.牙齿压入量，牙根吸收情况及牙槽骨改建的CBCT研究。每只Beagle犬分别在加力前及处死后拍摄CBCT，并将相关数据导入三维测量软件INVIVO5.0，对比分析实验侧与对照的牙齿压入量，牙根长度及根分叉变化、近远中牙槽骨高度变化，探究二者在牙齿压入、牙根吸收及牙槽骨改建方面的差异。 4.压入移动后根分叉下牙根之间特定区域牙槽骨骨小梁结构的Micro-CT检测。处死后取材，固定，适当修整后对骨块进行Micro-CT扫描，对比分析实验侧与对照侧根分叉下特定区域牙槽骨骨小梁的三维结构。 结果：1.本实验成功建立了骨皮质切开辅助组牙压入的动物实验模型，为后期的三维形态学检测，组织学及免疫组织化学分析奠定了基础。 2.大体测量的结果提示：2周时实验侧及对照侧的牙齿压入量无明显差异，其余时间点包括4、6、8、（P＜0.05）；10、12周余下动物实验侧的压入量均高于对照侧，提示骨皮质切开可加速组牙的压入移动。 3.CBCT测量结果提示：除两周组外，其余三组实验侧牙齿压入量均大于对照侧，差异有统计学意义（P＜0.05）。12周组近远中牙根吸收量（MRRDRR）实验侧小于对照侧，差异有统计学意义（P＜0.05）。8周组及12周组近远中牙槽骨高度降低量（MACDAC）实验侧大于对照侧（P＜0.05），但结合压入量进行分析，实验侧牙槽骨高度降低量占压入量的比例明显小于对照侧。实验侧及对照侧根分叉牙根吸收量均无明显差异。提示：骨皮质切开辅助牙齿压入移动可能减轻了牙根吸收的程度并使牙槽骨高度得到更好的保留。 4.Micro-CT结果提示：加力4周组及加力8周组骨小梁体积分数实验侧小于对照侧（P＜0.05）。另外，加力4周组实验侧骨小梁数目小于对照侧（P＜0.05）,加力8周组实验侧骨小梁间隙大于对照侧（P＜0.05），差异有统计学意义。其余各组实验侧及对照侧各测量指标之间无明显差异。 综上所述：骨皮质切开联合应用微种植体支抗可加速正畸牙的压入移动，并可获得更大的压入移动量。同时，辅助骨皮质切开可能在一定程度上减少了牙根吸收、并对维持牙齿周围骨量有积极作用。这可能与辅助骨皮质切开手术后牙槽骨骨改建更加活跃有关。
[Abstract]:The press-in movement of the teeth is one of the difficulties in the treatment of orthodontic treatment. In the clinical of orthodontics, the malocclusion of the molar due to the early loss of the tooth and the bone opening and closing of the molar is often caused by the press-in movement of the teeth in the posterior teeth. The lack of stable anchorage in the past is the main cause of the difficulty in the press-in of the group, and the appearance of the mini-screw implant MIA provides a good solution for the continuous press-in of the group teeth. However, it has been reported that the application of an implant-supported low-pressure molar leads to root-tip and root resorption in the root-bifurcation region. The osteotomy of the cortical bone around the teeth is performed to achieve the purpose of accelerating the movement of the tooth, having a small wound, a simple operation, and the like. Point. There are clinical reports that the use of a cortical cut-assisted microimplant-assisted microimplant support for molar depression can effectively speed up the speed of tooth movement The results of the present study on the animal experimental study on the accelerated orthodontic tooth movement in the bone cortex were mainly focused on the internal and sagittal movement of the anterior teeth, while the three-dimensional morphological study on the root resorption and the reconstruction of the alveolar bone was not reported. Therefore, it is necessary to establish an animal experiment model of the tooth-in movement of the bone-cortex cut-off auxiliary group. The comparative study uses the micro-implant support and the bone-cortical cut-in combination with the micro-implant support-assisted group to press in the tooth-down rate, the root resorption, and the alveolar bone remodeling. Objective: To establish a model of Beagle dog experimental animal model for bone-cortical cut-in and to study the three-dimensional morphological effect of cortical cut on the press-in effect of the group and to compare the three-dimension of the different time nodes in the longitudinal direction. Morphological changes, and the absorption and the alveolar bone of the tooth root in the press-in movement of the cortical cut Impact of alteration . Methods:1. To establish a bone-cortical cut-off auxiliary group of teeth. Animal experiment model.8 adult male Beagle dogs were randomly divided into two groups: 4 groups,2 in each group. The left and right side of the lower jaw of each Beagle dog were randomly assigned to the experimental side and the control side. The depression of P3, P4 and the four groups were 2 weeks,4 weeks,8 weeks and 12 weeks after the application of the booster, respectively. kill, get the specimen.2. Teeth Gross measurement of the rate of movement. The non-stressed micro-implant was implanted as a marker, and the amount of press-in movement of the experimental side and the control side was compared with that of the two-week, four-week,6-week,8-week,10-week, and 12-week tooth depression of the Beagle dog. Draw the time curve of the pressure and pressure, and compare and analyze the experimental side and the pair. the rate of tooth movement on the side of the tooth.3. tooth pressure, tooth root absorption and tooth CBCT was used to study the alteration of the alveolar bone. The CBCT was taken and the relevant data were introduced into the three-dimensional measurement software, INVIVO 5.0, before and after the application, and the height of the alveolar bone in the near-distal region was changed. To study the effect of the two in the tooth press and root resorption 4. The difference in the reconstruction of alveolar bone.4. The bone trabecula of the alveolar bone in the specific area between the root and the root in the root bifurcation after the press-in movement Micro-CT (Micro-CT) of the structure was performed. After being killed, the bone mass was scanned by the Micro-CT, and the contrast between the experimental side and the control-side root was specific. alveolar bone The three-dimensional structure of the bone trabecula was successfully established in this experiment. 2. The results showed that there was no significant difference between the experimental side and the control side in 2 weeks, and the remaining time points were 4,6,8, (P <0.05), and the pressure on the experimental side of the remaining animals in the 10 and 12 weeks was higher than that of the control. 3. The results of CBCT showed that the tooth pressure in the other three groups was higher than that of the control (P <0.05) except for the two-week group (P <0.05). The experimental side of the absorption amount (MRRDRR) was less than that of the control side (P <0.05). The experimental side of the alveolar bone height reduction (MACD) in the 8-week group and the 12-week group was higher than that of the control (P <0.05). However, the experimental side of the experimental-side alveolar bone was the same as that of the control side (P <0.05). and the ratio of the height-decreasing amount to the pressure-reducing agent is obviously smaller than that of the control side, and the experiment There was no significant difference in the absorption of root in the lateral and control side. The degree of absorption and the height of the alveolar bone were better preserved. The experimental side of the beam volume fraction was less than that of the control (P <0.05). In addition, the number of bone trabeculae in the experimental side of the 4-week group was less than that of the control (P <0.05). The difference was of statistical significance on the control side (P <0.05). The rest There was no significant difference between the measurement indexes of the experimental side and the control side of each group. In conclusion, the combined application of the micro-implant support to the fracture of the bone cortex It is possible to accelerate the press-in movement of the orthodontic tooth and to obtain a greater amount of press-in movement. reduces the absorption of the root and has a positive effect on the bone mass surrounding the teeth, which may
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5
本文编号：2432853
[Abstract]:The press-in movement of the teeth is one of the difficulties in the treatment of orthodontic treatment. In the clinical of orthodontics, the malocclusion of the molar due to the early loss of the tooth and the bone opening and closing of the molar is often caused by the press-in movement of the teeth in the posterior teeth. The lack of stable anchorage in the past is the main cause of the difficulty in the press-in of the group, and the appearance of the mini-screw implant MIA provides a good solution for the continuous press-in of the group teeth. However, it has been reported that the application of an implant-supported low-pressure molar leads to root-tip and root resorption in the root-bifurcation region. The osteotomy of the cortical bone around the teeth is performed to achieve the purpose of accelerating the movement of the tooth, having a small wound, a simple operation, and the like. Point. There are clinical reports that the use of a cortical cut-assisted microimplant-assisted microimplant support for molar depression can effectively speed up the speed of tooth movement The results of the present study on the animal experimental study on the accelerated orthodontic tooth movement in the bone cortex were mainly focused on the internal and sagittal movement of the anterior teeth, while the three-dimensional morphological study on the root resorption and the reconstruction of the alveolar bone was not reported. Therefore, it is necessary to establish an animal experiment model of the tooth-in movement of the bone-cortex cut-off auxiliary group. The comparative study uses the micro-implant support and the bone-cortical cut-in combination with the micro-implant support-assisted group to press in the tooth-down rate, the root resorption, and the alveolar bone remodeling. Objective: To establish a model of Beagle dog experimental animal model for bone-cortical cut-in and to study the three-dimensional morphological effect of cortical cut on the press-in effect of the group and to compare the three-dimension of the different time nodes in the longitudinal direction. Morphological changes, and the absorption and the alveolar bone of the tooth root in the press-in movement of the cortical cut Impact of alteration . Methods:1. To establish a bone-cortical cut-off auxiliary group of teeth. Animal experiment model.8 adult male Beagle dogs were randomly divided into two groups: 4 groups,2 in each group. The left and right side of the lower jaw of each Beagle dog were randomly assigned to the experimental side and the control side. The depression of P3, P4 and the four groups were 2 weeks,4 weeks,8 weeks and 12 weeks after the application of the booster, respectively. kill, get the specimen.2. Teeth Gross measurement of the rate of movement. The non-stressed micro-implant was implanted as a marker, and the amount of press-in movement of the experimental side and the control side was compared with that of the two-week, four-week,6-week,8-week,10-week, and 12-week tooth depression of the Beagle dog. Draw the time curve of the pressure and pressure, and compare and analyze the experimental side and the pair. the rate of tooth movement on the side of the tooth.3. tooth pressure, tooth root absorption and tooth CBCT was used to study the alteration of the alveolar bone. The CBCT was taken and the relevant data were introduced into the three-dimensional measurement software, INVIVO 5.0, before and after the application, and the height of the alveolar bone in the near-distal region was changed. To study the effect of the two in the tooth press and root resorption 4. The difference in the reconstruction of alveolar bone.4. The bone trabecula of the alveolar bone in the specific area between the root and the root in the root bifurcation after the press-in movement Micro-CT (Micro-CT) of the structure was performed. After being killed, the bone mass was scanned by the Micro-CT, and the contrast between the experimental side and the control-side root was specific. alveolar bone The three-dimensional structure of the bone trabecula was successfully established in this experiment. 2. The results showed that there was no significant difference between the experimental side and the control side in 2 weeks, and the remaining time points were 4,6,8, (P <0.05), and the pressure on the experimental side of the remaining animals in the 10 and 12 weeks was higher than that of the control. 3. The results of CBCT showed that the tooth pressure in the other three groups was higher than that of the control (P <0.05) except for the two-week group (P <0.05). The experimental side of the absorption amount (MRRDRR) was less than that of the control side (P <0.05). The experimental side of the alveolar bone height reduction (MACD) in the 8-week group and the 12-week group was higher than that of the control (P <0.05). However, the experimental side of the experimental-side alveolar bone was the same as that of the control side (P <0.05). and the ratio of the height-decreasing amount to the pressure-reducing agent is obviously smaller than that of the control side, and the experiment There was no significant difference in the absorption of root in the lateral and control side. The degree of absorption and the height of the alveolar bone were better preserved. The experimental side of the beam volume fraction was less than that of the control (P <0.05). In addition, the number of bone trabeculae in the experimental side of the 4-week group was less than that of the control (P <0.05). The difference was of statistical significance on the control side (P <0.05). The rest There was no significant difference between the measurement indexes of the experimental side and the control side of each group. In conclusion, the combined application of the micro-implant support to the fracture of the bone cortex It is possible to accelerate the press-in movement of the orthodontic tooth and to obtain a greater amount of press-in movement. reduces the absorption of the root and has a positive effect on the bone mass surrounding the teeth, which may
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5
【参考文献】
相关期刊论文 前2条
1 段银钟 ,陈华 ,张巧余 ,蔺崇甲;关于正畸力值与牙齿移动速度的实验研究[J];第四军医大学学报;1990年01期
2 胡轶;丁寅;李洁;宋超锋;杨文晔;;应用微型种植体支抗压低犬后牙过程中牙周组织改建的组织学研究[J];牙体牙髓牙周病学杂志;2008年03期
本文编号：2432853
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