iRoot BP对人乳牙牙髓细胞增殖、矿化的影响

发布时间：2019-03-15 09:53

【摘要】：目的探讨iRoot BP对人乳牙牙髓细胞增殖、矿化的影响,为其用于乳牙活髓保存治疗提供依据。方法将iRoot BP和三氧化矿物凝聚体(MTA)分别制成高度2 mm、直径8 mm的圆柱体,置于DMEM细胞培养基中,收集浸提液。原代培养人乳牙牙髓细胞并鉴定。随机将人乳牙牙髓细胞分为iRoot BP组、MTA组、对照组。iRoot BP组和MTA组分别加入适量iRoot BP、MTA浸提液,使其终浓度分别为10%、20%、50%、100%,对照组不予处理。采用CCK-8法检测三组培养24、72 h时细胞增殖指数,采用实时荧光定量PCR法检测三组培养48、96 h时碱性磷酸酶(ALP)mRNA表达(iRoot BP、MTA浸提液浓度均为10%)。结果与对照组同时间点比较,含50%、100%浸提液的iRoot BP组、MTA组细胞增殖指数均降低(P均0.05),而含10%、20%浸提液的iRoot BP组、MTA组细胞增殖指数无明显变化(P均0.05)。与对照组同时间点比较,iRoot BP组、MTA组ALP mRNA相对表达量均升高(P均0.05)。iRoot BP组与MTA组作用48、96 h ALP mRNA相对表达量比较差异均无统计学意义(P均0.05)。结论 10%iRoot BP浸提液对人乳牙牙髓细胞增殖无明显影响,但可明显促进细胞矿化。
[Abstract]:Objective To study the effect of iRoot BP on the proliferation and mineralization of human dental pulp cells. Methods The iRoot BP and the three-oxidized mineral aggregate (MTA) were respectively made into a cylinder with a height of 2 mm and a diameter of 8 mm, and placed in a DMEM cell culture medium to collect the extract. Primary cultured human dental pulp cells and identified. The human dental pulp cells were randomly divided into the iRoot BP group, the MTA group and the control group. The final concentrations of iRoot BP and MTA were 10%,20%,50% and 100% respectively in the iRoot BP and MTA groups, and the control group was not treated. The expression of alkaline phosphatase (ALP) mRNA (iRoot BP and MTA extract was 10%) was detected by real-time fluorescence quantitative PCR. Results Compared with the control group, the proliferation index of the iRoot BP group and the MTA group with 50% and 100% of the extract was lower (P <0.05), while the proliferation index of the iRoot BP group and the MTA group with 10% and 20% of the extract did not change significantly (P <0.05). At the same time point in the control group, the relative expression of ALP mRNA in the iRoot BP group and the MTA group was increased (P <0.05). There was no significant difference in the relative expression of 48 and 96 h ALP mRNA in the iRoot BP group and the MTA group (P 0.05). Conclusion The 10% iRoot BP extract has no significant effect on the proliferation of human dental pulp cells, but it can promote the mineralization of the cells.
【作者单位】：北京大学口腔医学院口腔医院第三门诊部;北京大学口腔医学院口腔医院;
【分类号】：R781

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