反复熔铸对非镍基烤瓷合金溶血性能及口腔粘膜刺激性影响的研究

发布时间：2019-04-07 18:24

【摘要】：目的：研究反复熔铸对钴铬、纯钛、钯基和金铂等四种非镍基烤瓷合金溶血性能及口腔粘膜刺激性的影响。方法：将上述4种烤瓷合金于真空加氩气下分别熔铸1~3次，得到1~3代测试试件。再次熔铸前，将废旧合金按“非镍基烤瓷合金再次熔铸前处理工艺”处理。（1）根据ISO/TR7405-1984标准，将4种烤瓷合金熔铸后，制成圆片状测试试件并打磨抛光，消毒灭菌后制备烤瓷合金浸提液。将试件浸提液依次加入生理盐水、新鲜抗凝兔血，离心，取上清液于分光光度计测试吸光度值，计算溶血率，用SPSS17.0统计软件对同种烤瓷合金3代间的溶血吸光度值进行方差分析。（2）根据YY-T0127.1-1993标准，将4种烤瓷合金经1~3次熔铸后，制成圆片状测试试件并打磨抛光，以聚丙烯为阴性对照材料，工业聚氯乙烯为阳性对照材料，制成相同大小的圆片状试件。将试件缝合固定于金黄地鼠的一侧颊囊粘膜，14天后观察与试件接触的颊囊粘膜情况并评分，同时切取该粘膜组织制作HE染色切片并进行病理评分。结果：（1）4种非镍基烤瓷合金经1~3次熔铸后，其溶血率均小于5%，同种烤瓷合金1~3代间溶血吸光度值的差异无统计学意义（P0.05）。（2）通过14天的观察发现,金黄地鼠活动、饮食正常；试验组局部粘膜与阴性对照组无明显差异，未见充血、出血、糜烂、溃疡等，阳性对照组粘膜表面出现了较明显的充血、红肿。显微镜下观察，试验组上皮完整、连续，细胞层次清晰，基底层细胞形态规则，呈矮柱状，无或少量炎症细胞浸润，组织轻度水肿，，与阴性对照组无显著差异。除了钴铬3代烤瓷合金对口腔粘膜的刺激性为轻度外，4种非镍基烤瓷合金经1~3次熔铸后，其对口腔粘膜的刺激为极轻度或无刺激。结论：钴铬、纯钛、钯基与金铂等4种非镍基烤瓷合金经过1～3次熔铸后，其溶血性能及口腔粘膜刺激性符合相关要求。
[Abstract]:Aim: to study the effects of repeated melting casting on hemolytic properties and oral mucosal irritation of four non-nickel-based porcelain alloys, such as cobalt-chromium, pure titanium, palladium-based and gold-platinum. Methods: the above four kinds of ceramic alloys were fused and cast in vacuum and argon for 1 ~ 3 times respectively, and the test specimens of 1 ~ 3 generations were obtained. Before re-casting, the waste alloys were treated according to the "pre-casting process for non-nickel-based porcelain alloys". (1) according to the ISO/TR7405-1984 standard, four kinds of ceramic alloys were fused and cast, and then made into wafer-shaped test specimens and polished. The ceramic alloy extract solution was prepared after disinfection and sterilization. The sample extract was added saline in turn, fresh anticoagulant rabbit blood was centrifuged, the supernatant was taken to test the absorbance value by spectrophotometer, and the hemolysis rate was calculated. The SPSS17.0 statistical software was used to analyze the variance of the hemolytic absorbance value of the same porcelain-to-porcelain alloy in the third generation. (2) according to the YY-T0127.1-1993 standard, four kinds of porcelain-fused-to-porcelain alloys were melted and cast for 3 times, and then the test specimens were made and polished. Using polypropylene as negative control material and industrial polyvinyl chloride as positive control material, circular specimen of the same size was made. The specimens were sutured to one side of the hamster buccal pouch mucosa. 14 days later, the buccal pouch mucosa in contact with the specimen was observed and scored. At the same time, the mucosa tissue was cut to make HE staining section and the pathological score was carried out. Results: (1) the hemolysis rate of 4 kinds of non-nickel-based porcelain alloys were all less than 5% after 3 times of melting and casting. There was no significant difference in hemolytic absorbance between the same porcelain-fused-to-metal alloys (P0.05). (2) after 14 days of observation, and there was no significant difference in the hemolytic absorbance between the first generation and the third generation of the same ceramic alloy (P0.05). Golden hamster activity, normal diet; There was no significant difference between the experimental group and the negative control group, but no congestion, bleeding, erosion, ulcer and so on appeared on the surface of the mucosa of the positive control group, and there was obvious hyperemia and redness on the surface of the mucous membrane in the positive control group. Under the microscope, the epithelium of the experimental group was intact and continuous, the cell level was clear, the basal cell morphology was regular, with low columnar shape, no or a few inflammatory cells infiltration, slight edema of the tissue, and there was no significant difference between the test group and the negative control group. Except for the mild irritation of Co-Cr 3 generation porcelain alloy to oral mucosa, the stimulation of 4 kinds of non-nickel-based porcelain-ceramic alloys on oral mucosa was very mild or non-irritating after 3 times melting and casting of 4 kinds of non-nickel-based porcelain alloys. Conclusion: the hemolytic properties and oral mucosal irritation of four kinds of non-nickel-based porcelain alloys, such as cobalt-chromium, pure titanium, palladium-based and gold-platinum, meet the relevant requirements after 1 ~ 3 times of melting and casting.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783

【参考文献】