人牙周膜成纤维细胞凋亡与体外高糖环境和脂多糖的交互作用

发布时间：2019-04-09 12:16

【摘要】：背景:已有研究表明,高糖和脂多糖在单独作用下都能够促进人牙周膜成纤维细胞的凋亡,但体外培养的人牙周膜成纤维细胞在高糖和脂多糖共同作用下的凋亡行为尚未有相关报道。目的:通过不同浓度脂多糖影响高糖环境下人牙周膜成纤维细胞,观察细胞增殖、凋亡以及凋亡相关基因Bax和Bcl-2表达的变化。方法:原代培养并鉴定人牙周膜成纤维细胞,5-8代用于后继实验。分别用葡萄糖(5.5,25 mmol/L)和脂多糖(0,1,10 mg/L)作用于细胞24 h及48 h。结果与结论:(1)在正常的葡萄糖浓度(5.5 mmol/L)下,质量浓度为10 mg/L的脂多糖能够显著抑制细胞的增殖(P0.05),促进细胞的凋亡(P0.05),Bax和Bcl-2 mR NA表达增强(P0.05),Bcl-2/Bax的比值显著降低(P0.05);(2)在高糖浓度(25 mmol/L)下,脂多糖诱导的细胞增殖的抑制、细胞凋亡、Bax和Bcl-2 mR NA的表达以及Bcl-2/Bax比值的降低都显著增强(P0.05);(3)方差分析结果显示,高糖和脂多糖在诱导细胞凋亡方面有显著的交互作用(P0.05);(4)结果说明,脂多糖在高糖环境下介导的人牙周膜成纤维细胞增殖的抑制、凋亡及凋亡相关基因Bax和Bcl-2的表达显著增强,脂多糖和高糖在细胞凋亡方面有显著的交互作用。
[Abstract]:Background: it has been shown that both high glucose and lipopolysaccharide can promote the apoptosis of human periodontal ligament fibroblasts. However, the apoptosis of human periodontal ligament fibroblasts cultured in vitro under the action of high glucose and lipopolysaccharide has not been reported. Aim: to observe the effects of lipopolysaccharide (LPS) on proliferation, apoptosis and the expression of apoptosis-related genes Bax and Bcl-2 in human periodontal ligament fibroblasts in high glucose environment. Methods: human periodontal ligament fibroblasts were cultured and identified. The cells were treated with glucose (5.5, 25 mmol/L) and lipopolysaccharide (0, 1, 10 mg/L) for 24 h and 48 h, respectively. Results & conclusion: (1) under normal glucose concentration (5.5 mmol/L), lipopolysaccharide (10 mg/L) could significantly inhibit cell proliferation (P0.05) and promote cell apoptosis (P0.05). The expression of Bax and Bcl-2 mR NA was increased (P0.05), and the ratio of Bcl-2/Bax was significantly decreased (P0.05). (2) under high glucose concentration (25 mmol/L), the inhibition of cell proliferation, apoptosis, the expression of Bax and Bcl-2 mR NA and the decrease of Bcl-2/Bax ratio were significantly increased (P0.05). (3) the results of ANOVA showed that there was significant interaction between high glucose and lipopolysaccharide in inducing apoptosis (P0.05). (4) the results showed that the inhibition of proliferation, apoptosis and the expression of apoptosis-related genes Bax and Bcl-2 in human periodontal ligament fibroblasts mediated by lipopolysaccharide in high glucose environment were significantly increased. Lipopolysaccharide and high glucose have significant interaction in cell apoptosis.
【作者单位】：潍坊医学院口腔医学院;青岛市市立医院口腔科;青岛大学医学院附属青岛市市立医院口腔医学中心;湖北医药学院附属东风口腔医院预防保健科;
【基金】：山东省医药卫生科技发展计划项目(2013WS0014)~~
【分类号】：R781

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