富自体浓缩生长因子纤维蛋白提取液对MC3T3-E1成骨细胞增殖与分化的影响

发布时间：2019-04-17 18:08

【摘要】：目的:提取富自体浓缩生长因子纤维蛋白(concentrated growth factors,CGF),研究CGF提取液对MC3T3-E1成骨细胞体外增殖、分化能力的影响。方法:制取CGF提取液,实验组采用CGF条件培养基,对照组为α-MEM完全培养基分别培养MC3T3-E1细胞,CCK-8法测定细胞增殖情况;ALP检测碱性磷酸酶活性;RT-PCR测定Runt相关转录因子-2(Runx2)和成骨细胞特异性转录因子(Osx)基因表达情况。结果:CCK-8显示各浓度CGF提取液有不同程度促增殖作用,其中10%CGF强于其他组;以10%CGF为实验浓度,ALP活性检测显示实验组3、5、7d活性增强;RT-PCR结果显示实验组Runx2和Osx的基因表达水平均明显增高。结论:CGF释放的生长因子可促进MC3T3-E1成骨细胞增殖、分化。
[Abstract]:Aim: to extract autogenous concentrated growth factor fibrin (concentrated growth factors,CGF) and study the effect of CGF extract on the proliferation and differentiation of MC3T3-E1 osteoblasts in vitro. Methods: CGF extract was prepared. MC3T3-E1 cells were cultured in CGF conditioned medium in experimental group and 伪-MEM complete medium in control group. The proliferation of MC3T3-E1 cells was measured by CCK- 8 method, alkaline phosphatase activity was measured by ALP, and alkaline phosphatase activity was measured by ALP. The expression of Runt-associated transcription factor-2 (Runx2) and osteoblast-specific transcription factor (Osx) was measured by RT-PCR. Results: CCK-8 showed that each concentration of CGF extract had the effect of promoting proliferation in varying degrees, in which 10%CGF was stronger than other groups, with 10%CGF as the experimental concentration, the activity of ALP in the experimental group was increased at 3,5,7 days after treatment, and there was no significant difference between the experimental group and the control group. The results of RT-PCR showed that the gene expression levels of Runx2 and Osx in the experimental group were significantly higher than those in the control group. Conclusion: CGF released growth factor can promote the proliferation and differentiation of MC3T3-E1 osteoblasts.
【作者单位】：西南医科大学口腔医学院;西南医科大学附属口腔医院;
【基金】：四川省科技厅-泸州市科技局-泸州医学院联合项目(编号:14JC0122-LY51)
【分类号】：R783.6

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