伊犁黑蜂蜂胶对口腔主要致龋细菌生物膜作用的实验研究

发布时间：2019-04-27 06:21

【摘要】：目的研究伊犁黑蜂蜂胶对口腔常见致龋细菌生物膜的影响,探讨其防龋效果及可能的防龋机制。方法通过结晶紫染色法测定黑蜂蜂胶对口腔常见致龋菌(变异链球菌、表兄链球菌、血链球菌、黏性放线菌、内氏放线菌)的最小生物膜清除浓度(MBEC);培养测试细菌24 h单菌生物膜,加入MBEC及以下的3个浓度配置成初始p H值为7.0的含药培养基,厌氧培养24 h后测p H值,并计算p H变化值以检测不同浓度黑蜂蜂胶对测试菌单菌生物膜产酸能力的影响。蒽酮法测定MBEC及以下的3个浓度的黑蜂蜂胶对变异链球菌24 h单菌生物膜产生水不溶性胞外多糖的影响。结果黑蜂蜂胶对变异链球菌、表兄链球菌、血链球菌、黏性放线菌、内氏放线菌的MBEC分别是6.25、1.56、3.13、0.78、0.78 mg·m L-1;黑蜂蜂胶可使各测试菌单菌生物膜Δp H降低,蜂胶各组与对照组相比差异均有统计学意义(P0.05);在MBEC浓度时,蜂胶可使变异链球菌合成水不溶性胞外多糖的能力降低。结论伊犁黑蜂蜂胶具有一定的防龋效果,其可能的防龋机制是通过有效清除口腔主要致龋细菌单菌生物膜,抑制测试菌株产酸、合成水不溶性胞外多糖的能力起作用的。
[Abstract]:Objective to study the effect of Yili wasp propolis on the bacterial biofilm of dental caries, and to explore its caries prevention effect and possible anti-caries mechanism. Methods the minimum biofilm clearance concentration (MBEC);) of wasp propolis on common oral caries-causing bacteria (Streptococcus mutans, Streptococcus sanguis, Actinomycetes viscosus, Actinomycetes Nei) was determined by crystal violet staining. The bacterial biofilm was cultured and tested for 24 h, and the initial pH value was 7.0.After anaerobic culture for 24 h, the pH value was measured by adding three concentrations of MBEC and below. The pH change value was calculated to detect the effect of different concentration of bee propolis on the acid production ability of the biofilm. The effects of three concentrations of MBEC and below three concentrations of bee propolis on the production of water-insoluble extracellular polysaccharides from the biofilm of Streptococcus mutans for 24 h were determined by anthrone method. Results the MBEC of propolis against Streptococcus mutans, Streptococcus sanguis, Actinomycetes actinomycetes was 6.25,1.56,3.13,0.78 mg 路mL ~ 1, respectively. Black bee propolis could decrease the 螖 pH of the biofilm of each tested bacteria, and there was significant difference between the propolis groups and the control group (P0.05). At the concentration of MBEC, the propolis could decrease the ability of synthesizing water insoluble extracellular polysaccharides from Streptococcus mutans. Conclusion Yili wasp propolis has some anti-caries effect. The possible mechanism of caries prevention is that it can effectively eliminate the single bacterial biofilm of caries-causing bacteria in oral cavity, inhibit the acid production of tested strains, and synthesize water-insoluble extracellular polysaccharides.
【作者单位】：新疆医科大学第一附属医院牙体牙髓病科·新疆维吾尔自治区口腔医学研究所;
【基金】：新疆地方病分子生物学实验室开放课题基金资助项目(XJDX0208-2011-06)
【分类号】：R780.2

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