正丁酸钠对涎腺腺样囊性癌细胞株ACC-2侵袭、迁移的影响及机制研究

发布时间：2019-05-05 05:53

【摘要】：目的探讨不同浓度正丁酸钠对涎腺腺样囊性癌细胞株ACC-2侵袭、迁移的影响与其作用机制。方法噻唑蓝(MTT)法探索正丁酸钠作用ACC-2细胞的最佳浓度,Transwell小室实验检测正丁酸钠对ACC-2细胞侵袭、迁移能力的影响,Western blot和实时荧光定量PCR(RT-PCR)分别检测5组浓度药物作用后ACC-2细胞中高迁移率族蛋白1(HMGB1)、TLR4的mRNA和蛋白的表达。结果与对照组相比,0.625、1.250、2.500、5.000、10.000 mmol/L 5组浓度的正丁酸钠均能抑制ACC-2细胞增殖(P0.05)且呈明显浓度依赖性;5组浓度正丁酸钠对细胞侵袭能力的影响差异无统计学意义(P0.05);2.500、5.000、10.000mmol/L浓度正丁酸钠可以抑制ACC-2细胞的迁移能力(P0.05),同时降低ACC-2细胞HMGB1、TLR4的mRNA及蛋白表达(P0.05);相关性分析显示TLR4蛋白表达的降低与HMGB1的抑制呈正相关(r=0.810,P0.05)。结论正丁酸钠能够抑制ACC-2细胞增殖,浓度较高时可以抑制ACC-2细胞的迁移能力,同时降低HMGB1、TLR4的mRNA与蛋白表达,提示NaB对ACC-2迁移能力抑制可能通过下调HMGB1、TLR4的mRNA及蛋白表达来实现。
[Abstract]:Aim to investigate the effects of sodium butyrate on the invasion and migration of salivary adenoid cystic carcinoma cell line ACC-2 and its mechanism. Methods the optimal concentration of sodium butyrate on ACC-2 cells was investigated by thiazole blue (MTT) assay. The effect of sodium butyrate on the invasion and migration of ACC-2 cells was detected by Transwell chamber assay. Western blot and real-time fluorescence quantitative PCR (RT-PCR) were used to detect the expression of high mobility group protein 1 (HMGB1), mRNA and protein of TLR4 in ACC-2 cells treated with 5 groups of drugs respectively. Results compared with the control group, sodium butyrate at the concentration of 0.625,1.250,2.500,5.000,10 000 mmol/L could inhibit the proliferation of ACC-2 cells (P0.05) in a significant concentration-dependent manner. There was no significant difference in the effect of sodium butyrate on the invasion ability of the cells in the five groups (P0.05). 2.500,5.000,10.000mmol / L sodium butyrate inhibited the migration of ACC-2 cells (P0.05) and decreased the mRNA and protein expression of HMGB1,TLR4 in ACC-2 cells (P0.05). Correlation analysis showed that the decrease of TLR4 protein expression was positively correlated with the inhibition of HMGB1 (r = 0.810, P0.05). Conclusion Sodium butyrate can inhibit the proliferation of ACC-2 cells. At higher concentrations, sodium butyrate can inhibit the migration of ACC-2 cells and the expression of mRNA and protein in HMGB1,TLR4. It is suggested that the inhibition of NaB on ACC-2 migration may be due to the down-regulation of HMGB1,. The expression of mRNA and protein of TLR4 was realized.
【作者单位】：遵义医学院口腔组织病理学教研室;贵州省贵阳市口腔医院预防科;
【基金】：遵义市汇川区科技局红花岗科技局项目[遵红科合社字(2013)16号] 遵义医学院研究生教育创新计划项目[遵研创合CX字(2013)13号]
【分类号】：R739.8

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