饥饿状态对白念菌生物膜及滞留菌形成的影响

发布时间：2019-05-05 18:59

【摘要】：目的:探讨饥饿环境对白念菌生物膜形成以及其中耐药滞留菌形成的影响。方法 :体外分别构建对数期、稳定期和饥饿态白念菌24 h生物膜,两性霉素B(Amphotericin B)冲击24 h,冲击前、后行活菌菌落计数(CFU),计算得出滞留菌比例并进行统计学分析,同时以共聚焦显微镜观察和分析各组生物膜形成状况。采用SPSS11.70软件包对数据进行统计学分析。结果:对数期、稳定期和饥饿态白念菌生物膜形成能力依次下降(P0.05),具有显著差异,前2组形成菌丝相生物膜,而饥饿态菌形成酵母相生物膜。药物作用后,滞留菌比例依次升高(P0.05),具有显著差异。结论:营养状态对白念菌滞留菌的产生有影响,饥饿状态能够提高滞留菌的产生比例。
[Abstract]:Objective: to investigate the effect of starvation environment on biofilm formation of Candida albicans and the formation of drug-resistant retention bacteria. Methods: logarithmic, stable and hungry Candida albicans biofilms were constructed in vitro for 24 h. Amphotericin B (Amphotericin B) was incubated for 24 h, and then the viable colony count (CFU),) was performed before and after the shock. The proportion of residual bacteria was calculated and analyzed statistically, and the biofilm formation was observed and analyzed by confocal microscope. The data were analyzed by SPSS11.70 software package. Results: the biofilm formation ability of Candida albicans in logarithmic phase, stable phase and hungry state decreased sequentially (P0.05), with significant difference. The first two groups formed hyphal biofilm, while hungry bacteria formed yeast biofilm. After drug treatment, the percentage of retention bacteria increased in turn (P0.05), with significant difference. Conclusion: nutritional status has an effect on the production of Candida albicans, and starvation can increase the proportion of retention bacteria.
【作者单位】：山东大学口腔医学院牙体牙髓病科山东省口腔生物医学重点实验室;
【基金】：国家自然科学基金(30973310,81371158)~~
【分类号】：R780.2

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