大鼠下颌下腺细胞培养及其生物学特性研究

发布时间：2019-05-17 16:51

【摘要】：目的:体外培养大鼠下颌下腺细胞,并对其生物学特性进行研究,为涎腺疾病的探索提供体外模型。方法:无菌条件下取7d龄Wistar大鼠双侧下颌下腺,仔细分离脂肪、包膜、神经和血管,采用组织块培养法进行培养。运用酶消化法和差速贴壁法纯化细胞;免疫组化SP法检测Cytokeratin-8(CK-8)抗体和α-Amylase抗体的表达;PAS染色法检测细胞糖原分泌;扫描电镜(scanning electronic microscopy,SEM)观察细胞的形态学特征。结果:组织块培养法可成功获得下颌下腺细胞,免疫组化染色可见大部分细胞胞质为棕黄色,提示CK-8抗体表达阳性,α-Amylase抗体表达阳性;PAS染色可见胞质呈紫红色;SEM可见细胞伸出长短不一的伪足,胞核着色深,有些细胞可见分泌颗粒。结论:组织块培养法可以成功培养大鼠下颌下腺细胞,并且操作简便。
[Abstract]:Objective: To study the biological characteristics of submandibular gland cells in vitro, and to provide an in vitro model for the exploration of salivary gland diseases. Methods: The submandibular gland of 7 days old Wistar rats was taken under the condition of sterility, and the fat, the envelope, the nerves and the blood vessels were carefully separated, and the culture was carried out by the tissue culture method. The cells were purified by an enzyme digestion method and a differential adhesion method. The expression of the antibody of Cytokeratin-8 (CK-8) and the antigen-Aylase was detected by SP method. The cellular glycogen secretion was detected by PAS staining. The morphological characteristics of the cells were observed by scanning electron microscopy (SEM). Results: The submandibular gland cells were successfully obtained by the tissue culture method. The cytoplasm of most of the cells was brown yellow, and the expression of CK-8 was positive, and the expression of the antigen-Aylase was positive. PAS staining showed that the cytoplasm was purplish red, and the SEM showed that the cells extended out of the pseudo-foot with different lengths. The nuclei are colored deep and some cells are visible in the secretory granules. Conclusion: The submandibular gland cells of the rat can be cultured successfully by the tissue culture method, and the operation is simple and convenient.
【作者单位】：吉林大学口腔医学院;吉林省牙发育及颌骨重塑与再生重点实验室;
【基金】：吉林省直厅局项目(编号:5139073431)
【分类号】：R782

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