胰岛素样生长因子-1对骨质疏松大鼠种植体周围骨组织的影响

发布时间：2019-05-23 22:49

【摘要】：目的研究胰岛素样生长因子(IGF)-1局部应用对骨质疏松(OP)状态下种植体骨结合效果的影响。方法采用4个月龄SD雌性大鼠行卵巢摘除术建立OP模型,并以未切除卵巢的大鼠作为对照(假手术组)。建模成功后,切除卵巢的大鼠随机分为IGF-1组,OP组和雌二醇(E2)组,于右侧股骨远心端植入纯钛种植体,IGF-1组种植窝内局部应用含10μg重组人胰岛素样生长因子(rh IGF)-1的明胶海绵,其余各组种植窝内放入空白明胶海绵。E2组皮下注射给予苯甲酸E2。术后8 w分别处死动物取股骨,制作脱钙切片,形态计量学观察种植体周结合骨板宽度、骨小梁宽度和骨小梁面积百分比。结果 IGF-1组种植体周结合骨板厚度、骨小梁宽度和骨小梁面积百分比显著高于OP组,骨板结合宽度与E2组及假手术组无显著差异,骨小梁宽度和骨小梁面积百分比与E2组无差异但显著低于假手术组。结论 IGF-1局部应用可增加种植体周围骨板厚度,骨板外周骨小梁宽度和骨小梁面积/视野面积百分比,有助于改善OP状态下的骨结合。
[Abstract]:Objective to study the effect of insulin-like growth factor (IGF)-1 on bone binding in osteoporotic (OP). Methods OP model was established by ovariectomy in 4-month-old SD female rats, and the unresected rats were used as control group (pseudo-operation group). After successful modeling, ovariectomy rats were randomly divided into three groups: IGF-1 group, OP group and E2 group. Pure titanium implants were implanted at the distal end of the right thigh. In IGF-1 group, gelatin sponge containing 10 渭 g recombinant human insulin-like growth factor (rh IGF)-1 was used locally, and blank gelatin sponge was put into the implantation nest of the other groups. Benzoic acid E2 group was injected subcutaneously with benzoic acid E2. At 8 weeks after operation, the femurs were killed and decalcified sections were made. The width of bone plate, the width of bone cerebellum and the percentage of bone trabecular area were observed by morphometrics. Results the thickness of periimplantation bone plate, the width of bone trabecula and the percentage of bone trabecular area in IGF-1 group were significantly higher than those in OP group, but there was no significant difference between the bone plate binding width and E2 group and false operation group. The width of bone cerebellum and the percentage of bone trabecular area in E2 group were not different from those in E2 group, but significantly lower than those in sham operation group. Conclusion the local application of IGF-1 can increase the thickness of peridental bone plate, the width of perioskeleton and the percentage of bone trabecular area / visual field area, which is helpful to improve the bone union in OP state.
【作者单位】：西安医学院口腔医学系;
【基金】：陕西省科技厅资助项目(2015SF242) 陕西省教育厅科研课题(12JK0757) 西安医学院校级课题(2009FZ6)
【分类号】：R580;R783.6

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