五倍子水提取物对人牙髓细胞碱性磷酸酶及矿化的影响

发布时间：2019-05-26 18:56

【摘要】：目的:观察不同浓度的五倍子水提取物对人牙髓细胞碱性磷酸酶(ALP)活性及矿化能力的影响。方法:组织块酶消化法体外培养人牙髓细胞,并分别与不同浓度的五倍子水提取物(2.5、5、10、20、40μg/m L)共同培养;于培养48 h后,采用酶组织化学法检测各组细胞的ALP活性。并根据ALP实验结果筛选出(10、20μg/m L)五倍子水提取物浓度组,与牙髓细胞共同培养30 d后,用Von Kossa染色法观察各组细胞的生长及矿化结节形成情况。结果:与对照组相比,2.5、5μg/m L的五倍子水提取物对人牙髓细胞的ALP活性无显著性影响(P0.05),而浓度为10、20、40μg/m L的五倍子水提取物均可明显抑制牙髓细胞的ALP活性(P0.05);Von Kossa染色结果显示,五倍子水提取物与人牙髓细胞共同培养30 d后,可促进其矿化结节的形成。结论:五倍子水提取物可在一定程度上改变人牙髓细胞的碱性磷酸酶活性及矿化程度。
[Abstract]:Aim: to observe the effect of different concentrations of Galla chinensis water extract on alkaline phosphatase (ALP) activity and mineralization ability of human dental pulp cells. Methods: human dental pulp cells were cultured in vitro by tissue block enzyme digestion and co-cultured with different concentrations of gallnut water extract (2.5, 5, 10, 20, 40 渭 g / m L). After 48 hours of culture, the ALP activity of each group was detected by enzyme histochemical method. According to the results of ALP test, the water extract group of Galla chinensis (10,20 渭 g / m L) was selected and co-cultured with dental pulp cells for 30 days. The growth of cells and the formation of mineralization nodules in each group were observed by Von Kossa staining. Results: compared with the control group, 2.5 渭 g / mL Galla chinensis water extract had no significant effect on the ALP activity of human dental pulp cells (P 0.05). The water extract of Galla chinensis at the concentration of 10, 20 and 40 渭 g / mL could significantly inhibit the ALP activity of dental pulp cells (P 0.05). The results of Von Kossa staining showed that the water extract of Galla chinensis could promote the formation of mineralization nodules after co-culture with human dental pulp cells for 30 days. Conclusion: the water extract of Galla chinensis can change the alkaline phosphatase activity and mineralization degree of human dental pulp cells to a certain extent.
【作者单位】：第四军医大学口腔医院牙体牙髓病科军事口腔医学国家重点实验室;解放军第96375部队44卫生队;第四军医大学口腔医院修复科军事口腔医学国家重点实验室;解放军477医院口腔科;
【分类号】：R781

【参考文献】