芹菜素对变异链球菌致龋力影响的实验研究

发布时间：2019-05-28 06:57

【摘要】：目的:研究芹菜素(apigenin)对变异链球菌致龋力的影响。方法:用含20 g/L蔗糖的TPY液体培养基,以二倍体稀释法将芹菜素配制成12.5、25、50、100、200、4 00μg/mL的含药培养基,并与变异链球菌ATCC25175(血清C型)共同培养;分别观察不同浓度芹菜素对变异链球菌增殖及其产酸能力、粘附能力、葡聚糖转移酶(GTF)活力和水不溶性胞外多糖生成能力的影响。结果:与空白对照组相比,不同浓度芹菜素对变异链球菌的增殖和产酸均无明显影响(P0.05);但可明显抑制变异链球菌的粘附能力、GTF的活性以及水不溶性胞外多糖的生成量(P0.05);且随着芹菜素浓度的增加,其抑制作用逐渐增强,各浓度组相比,除200μg/mL与400μg/mL组间无统计学差异(P0.05)外,其他各浓度组间差异均有统计学意义(P0.05)。结论:芹菜素对变异链球菌的生长、产酸无影响,但对其粘附、GTF活性和水不溶性胞外多糖的生成具有抑制作用。
[Abstract]:Objective: to study the effect of apigenin (apigenin) on caries induced by Streptococcus mutans. Methods: TPY liquid medium containing 20 g 路L sucrose was used to prepare apigenin into 12.5, 25, 50, 200, 400 渭 g / mL medium by diploid dilution method, and co-cultured with Streptococcus mutans ATCC25175 (serum C). The effects of different concentrations of apigenin on the proliferation, acid production, adhesion, glucosyltransferase (GTF) activity and water insoluble extracellular polysaccharide production of Streptococcus mutans were observed. Results: compared with the blank control group, different concentrations of apigenin had no significant effect on the proliferation and acid production of Streptococcus mutans (P 0.05). However, it could significantly inhibit the adhesion ability of Streptococcus mutans, the activity of GTF and the production of water-insoluble extracellular polysaccharide (P 0.05). With the increase of apigenin concentration, the inhibitory effect increased gradually. There was no significant difference between 200 渭 g / mL and 400 渭 g / mL groups (P 0.05), but there was significant difference among the other concentration groups (P 0.05). Conclusion: apigenin has no effect on the growth and acid production of Streptococcus mutans, but it has inhibitory effect on its adhesion, GTF activity and the formation of water-insoluble extracellular polysaccharide.
【作者单位】：郑州大学口腔医学院;
【分类号】：R780.2

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