一氧化碳释放分子-2对实验性牙周炎大鼠动脉粥样硬化的作用
发布时间：2019-06-21 00:06
【摘要】：研究目的： 牙周炎是一种细菌感染为主多种因素共同作用下的慢性感染性疾病,其发生发展与全身多种疾病有着密切的关系。近年来牙周炎与动脉粥样硬化的关系受到日益关注,其中,牙周致病菌诱发的免疫炎性反应是牙周炎与动脉粥样硬化具有相关性的生物学基础,血清C反应蛋白(C react protein,CRP)、可溶性血管细胞黏附分子1(soluble vascular cell adhesion molecule-1,sVCAM-1)及氧化型低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)的水平已被发现与动脉粥样硬化的发生发展呈正相关。一氧化碳释放分子(CORM)是近年来合成的一种化合物,具有抗炎、调节白细胞粘附、舒张血管及抗血管内皮细胞凋亡等多种作用。在本研究中,我们以实验性牙周炎大鼠为动物实验模型,拟研究CORM对牙周炎大鼠血清中动脉粥样硬化相关指标的调节作用,初步探讨CORM对牙周炎个体动脉粥样硬化形成的影响,以期为牙周炎及其相关动脉粥样硬化症的治疗提供实验基础和理论依据。 研究方法： 1.动物的选择及分组：选择雄性6周龄wistar大鼠40只(200±20g)。随机分组分为正常组、牙周炎组(CP组)、一氧化碳干预组(CORM组)以及DMSO作用组。其中,CP组和CORM组采用丝线结扎同时局部注射内毒素法建立牙周炎模型。CP组和CORM组自结扎当天起分别注射等体积无菌生理盐水和一氧化碳释放分子-2(CORM-2,10mg/kg/d),DMSO组同天起注射等体积DMSO+生理盐水。注射时间连续4周。 2.实验室检测：分别于结扎后第1d、3d、7d、14d及28d,经大鼠颈静脉采血,ELISA方法检测血清中CRP,sVCAM-1,ox-LDL及IL-10含量。28d后取大鼠降主动脉进行油红O染色,观察各组脂质沉积情况。 3.统计学分析：采用SPSS17.0统计软件进行检验,以P0.05为差异有统计学意义。 结果： 1.对牙周炎炎症的抑制作用 (1)临床观察结果： CORM组及CP组大鼠的第二磨牙在结扎丝线后牙颈部有菌斑堆积；一周后,牙龈红肿且探诊出血,可探及牙周袋；14天后CP组和CORM组大鼠中分别有80%及10%个体的第二磨牙松动度达Ⅱ～Ⅲ度。 (2)大体标本观测结果：建模大鼠在4周后牙槽骨出现不同程度的吸收,CP组牙槽骨吸收量为2.47±0.31mm,高于CORM组2.05±0.22mm的牙槽骨吸收量(P0.05)。 (3)组织学观察：正常组及DMSO组中牙周组织完整,几乎无炎性细胞浸润。而CORM组及CP组中牙周组织内炎细胞浸润程度为2的分别占25%和85%。 2.血清学检验结果 (1)CRP,ox-LDL及sVCAM-1水平检验结果：建模第3天始,CP组大鼠的CRP和sVCAM-1水平出现较持续性的增强,显著高于CORM组(P0.05)。CORM组的ox-LDL水平在建模后第一天显著高于正常组和DMSO组(P0.05),但显著低于CP组(P0.05)。14天后,CP组的ox-LDL仍维持在高水平,但CORM组含量降至与正常组和DMSO组相近水平。 (2)IL-10水平检验结果：CORM组大鼠血清中IL-10水平在前7天内稳定上升并达到峰值,随后逐渐下降,但含量明显高于其他各组。(P0.05) 3.血管脂质沉积结果 CP组中60%的大鼠动脉内膜可见橙红色脂质染色,CORM组及其他两组中均未发现脂质沉积。 结论： 1.CORM-2在抑制实验性大鼠牙周炎中牙周组织的炎症反应及牙槽骨吸收量的同时,降低了实验性大鼠牙周炎模型中动脉粥样硬化相关分子的水平,并显著提高抗炎因子IL-10的表达,从而降低了大鼠降主动脉的脂质沉积,对动脉粥样硬化在牙周炎大鼠中的发生发展有一定的影响。 2.CORM-2在抑制实验性牙周炎大鼠牙周炎性反应的同时能够对动脉粥样硬化的发生发展有一定的抑制作用,为牙周病患者中动脉粥样硬化的预防和治疗提供了新的思路和方法。
[Abstract]:The purpose of the study: Periodontitis is a chronic infectious disease, which is a kind of bacterial infection, which is a kind of bacterial infection. The development of periodontitis is closely related to various diseases of the whole body. In recent years, the relationship between periodontitis and atherosclerosis is of increasing concern, among which, the inflammatory response induced by periodontal pathogens is the biological basis of the correlation between periodontitis and atherosclerosis, and the serum C-reactive protein (CRP) The levels of soluble vascular cell adhesion molecule 1 (sVCAM-1) and oxidized low density lipoprotein (ox-LDL) have been found to be normal to the development of atherosclerosis The carbon monoxide releasing molecule (CORM) is a compound synthesized in recent years. It has many functions such as anti-inflammation, regulating leukocyte adhesion, relaxing blood vessel and anti-vascular endothelial cell apoptosis. In this study, we used experimental periodontitis as animal experimental model to study the effect of CORM on the related indexes of atherosclerosis in the serum of periodontitis rats, and to study the effect of CORM on the formation of atherosclerosis in the patients with periodontitis. In order to provide experimental basis and theoretical basis for the treatment of periodontitis and its associated atherosclerosis. According to. Research Method:1. Selection and grouping of animals: selection of 40 male 6-week-old Wistar rats (200 (20 g). The randomization was divided into the normal group, the periodontitis group (CP group), the carbon monoxide intervention group (CORM group), and the DM. The group of SO action, in which the CP group and the CORM group were established by the local injection of endotoxin at the same time. Periodontitis model. The CP group and the CORM group were injected with equal volume of sterile saline and carbon monoxide releasing molecule-2 (CORM-2,10 mg/ kg/ day) from the day of ligation, and the DMSO group was injected with the same volume of DMSO as the day. + Saline. Injection 2. Laboratory test: The serum CRP, sVCAM-1, ox-LDL and IL-10 were detected by the method of blood collection and ELISA in the rat jugular vein after the ligation. Group lipid deposition.3. Statistical analysis: SPSS17.0 statistical software was used for the test, with the exception of P0.05. to be a difference There is a statistical significance. Results: 1. Inflammation of the periodontitis Inhibition (1) Clinical Observations: The second molar in the CORM group and the group of CP rats had a plaque build-up after the ligation of the silk thread; one week later In the 14-day CP group and in the CORM group,80% and 1 were found. The second molar loosening degree of 0% of the individual was up to the degree of 鈪，
本文编号：2503628
[Abstract]:The purpose of the study: Periodontitis is a chronic infectious disease, which is a kind of bacterial infection, which is a kind of bacterial infection. The development of periodontitis is closely related to various diseases of the whole body. In recent years, the relationship between periodontitis and atherosclerosis is of increasing concern, among which, the inflammatory response induced by periodontal pathogens is the biological basis of the correlation between periodontitis and atherosclerosis, and the serum C-reactive protein (CRP) The levels of soluble vascular cell adhesion molecule 1 (sVCAM-1) and oxidized low density lipoprotein (ox-LDL) have been found to be normal to the development of atherosclerosis The carbon monoxide releasing molecule (CORM) is a compound synthesized in recent years. It has many functions such as anti-inflammation, regulating leukocyte adhesion, relaxing blood vessel and anti-vascular endothelial cell apoptosis. In this study, we used experimental periodontitis as animal experimental model to study the effect of CORM on the related indexes of atherosclerosis in the serum of periodontitis rats, and to study the effect of CORM on the formation of atherosclerosis in the patients with periodontitis. In order to provide experimental basis and theoretical basis for the treatment of periodontitis and its associated atherosclerosis. According to. Research Method:1. Selection and grouping of animals: selection of 40 male 6-week-old Wistar rats (200 (20 g). The randomization was divided into the normal group, the periodontitis group (CP group), the carbon monoxide intervention group (CORM group), and the DM. The group of SO action, in which the CP group and the CORM group were established by the local injection of endotoxin at the same time. Periodontitis model. The CP group and the CORM group were injected with equal volume of sterile saline and carbon monoxide releasing molecule-2 (CORM-2,10 mg/ kg/ day) from the day of ligation, and the DMSO group was injected with the same volume of DMSO as the day. + Saline. Injection 2. Laboratory test: The serum CRP, sVCAM-1, ox-LDL and IL-10 were detected by the method of blood collection and ELISA in the rat jugular vein after the ligation. Group lipid deposition.3. Statistical analysis: SPSS17.0 statistical software was used for the test, with the exception of P0.05. to be a difference There is a statistical significance. Results: 1. Inflammation of the periodontitis Inhibition (1) Clinical Observations: The second molar in the CORM group and the group of CP rats had a plaque build-up after the ligation of the silk thread; one week later In the 14-day CP group and in the CORM group,80% and 1 were found. The second molar loosening degree of 0% of the individual was up to the degree of 鈪，
本文编号：2503628
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