不同质量浓度釉基质蛋白培养人牙周膜细胞的生物活性

发布时间：2019-06-25 19:40

【摘要】：背景:大量研究证实釉基质蛋白可促进成骨细胞和成牙骨质细胞的再生,将其运用于牙周缺损治疗可达到接近生理性的牙周再生。目的:观察不同质量浓度釉基质蛋白对人牙周膜细胞增生、分化和迁移的影响。方法:取第3代人牙周膜细胞,以含不同质量浓度釉基质蛋白(0,12.5,25,50,100,250 mg/L)的无血清DMEM培养基培养。培养24 h后,采用3H-胸腺嘧啶核苷掺入法检测细胞增殖,MTT法检测细胞活性;培养48 h后,检测细胞碱性磷酸酶活性及骨钙素分泌;待细胞融合为单层,去除细胞培养液,以移液管头将单层细胞制备出1 mm宽的细胞切口,持续24 h观察细胞融合情况。结果与结论:当釉基质蛋白质量浓度在0-100 mg/L范围内,随着其质量浓度的升高,细胞增殖、活性、碱性磷酸酶活性、骨钙素分泌均逐渐升高,以100 mg/L升高最明显;当釉基质蛋白质量浓度增至250 mg/L时,细胞增殖、活性、碱性磷酸酶活性、骨钙素分泌均有所下降,但仍高于0 mg/L组。100 mg/L组在初始观察6 h时,创缘周围的细胞开始向中心生长,待培养12 h时,创缘两侧细胞开始融合,培养20 h后创缘两侧细胞融合完全创缘完全关闭完全,创面愈合优于其他质量浓度组。结果表明釉基质蛋白具有促进牙周膜细胞增殖、分化与迁移的能力。
[Abstract]:Background: a large number of studies have confirmed that ameloblast can promote the regeneration of osteoblasts and osteoblasts, and its application in the treatment of periodontal defects can achieve near physiological periodontal regeneration. Aim: to observe the effects of different concentrations of ameloblast on proliferation, differentiation and migration of human periodontal ligament cells. Methods: the third generation of human periodontal ligament cells were cultured in serum-free DMEM medium containing different concentrations of amellae matrix protein (0, 12.5, 25, 50100250 mg/L). After 24 hours of culture, cell proliferation was detected by 3H-thymidine incorporation assay, cell activity was detected by MTT assay, alkaline phosphatase activity and osteocalcin secretion were detected after 48 hours of culture, cell fusion into monolayer was removed, 1 mm wide cell incision was prepared with liquid transfer tube head, and cell fusion was observed for 24 hours. Results and conclusion: when the concentration of amel matrix protein was in the range of 0 鈮，

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