复合羟固醇无机小牛骨块修复犬下颌骨临界骨缺损的组织学研究
发布时间：2019-07-01 20:12
【摘要】：目的比较复合羟固醇无机小牛骨块(羟固醇骨块)和无机小牛骨粉在比格犬下颌骨临界骨缺损动物模型早期愈合阶段的成骨效应。方法选取健康雄性比格犬4只,拔除双侧下颌第3和第4前磨牙以及第1磨牙(P3-M1)后,双侧下颌骨各复制2个5 mm×10 mm×10 mm标准化箱型临界骨缺损。4周愈合期后,按半口对照设计,随机将羟固醇骨块和无机小牛骨粉植入骨缺损。术后4周处死,切取标本制作石蜡和硬组织切片,行组织学观察及组织形态学测量。结果实验动物愈合良好,羟固醇骨块组及无机小牛骨粉组均见临界骨缺损边缘及骨材料-宿主骨交界处明显的成骨细胞及新骨形成。HE染色组织形态学测量结果显示羟固醇骨块组和无机小牛骨粉组新生骨面积比分别为(37.22±2.16)%和(34.70±2.66)%,两者比较差异无统计学意义(P0.05);羟固醇骨块组成骨细胞数[(31.13±2.85)]与无机小牛骨粉组[(24.88±2.95)]比较,差异有统计学意义(P0.05)。硬组织切片亚甲基蓝-酸性品红染色组织形态学测量结果显示羟固醇骨块及无机小牛骨粉骨材料新生骨面积相对百分比分别为(20.13±1.32)%和(19.28±0.82)%,骨材料相对存留率分别为(30.80±1.16)%和(29.69±1.12)%,两者比较差异无统计学意义(P0.05)。结论羟固醇骨块在犬下颌骨临界骨缺损早期骨愈合阶段具有类似于小牛骨粉的成骨效果。由于羟固醇骨块具有良好的空间维持能力,更适合于临床大面积骨缺损的修复。
[Abstract]:Objective to compare the osteogenic effects of compound hydroxysterol inorganic calf bone mass (hydroxysterol bone mass) and inorganic calf bone powder in the early healing stage of mandibular critical bone defect in Beagle dogs. Methods four healthy male Beagle dogs were selected. After bilateral mandibular third and fourth premolars and first molar (P3-M1) were removed, two 5 mm 脳 10 mm standardized box critical bone defects were replicated in each side of the jaw. After 4 weeks of healing, the hydroxysterol bone mass and inorganic calf bone powder were randomly implanted into bone defects according to half mouth control design. Four weeks after operation, the specimens were cut to make paraffin and hard tissue sections, and the histology and histomorphology were observed. Results the experimental animals healed well. There were obvious osteoblasts and new bone formation at the edge of critical bone defect and the junction of bone material and host bone in the hydroxysterol bone mass group and the inorganic calf bone powder group. The histomorphology of HE staining showed that the ratio of new bone area in the hydroxysterol bone mass group and the inorganic calf bone powder group was (37.22 卤2.16)% and (34.70 卤2.66)%, respectively, and there was no significant difference between the two groups (P 0.05). The number of bone cells composed of hydroxysterol bone mass [(31.13 卤2.85)] was significantly different from that of inorganic calf bone powder group [(24.88 卤2.95)] (P 0.05). The relative percentage of new bone area of hydroxysterol bone mass and inorganic calf bone powder was (20.13 卤1.32)% and (19.28 卤0.82)%, respectively, and the relative retention rate of bone material was (30.80 卤1.16)% and (29.69 卤1.12)%, respectively. there was no significant difference between the two groups (P 0.05). The results showed that the relative percentage of new bone area was (20.13 卤1.32)% and (19.28 卤0.82)%, and the relative retention rate of bone material was (30.80 卤1.16)% and (29.69 卤1.12)%, respectively. Conclusion the osteogenic effect of hydroxysterol bone mass is similar to that of calf bone powder in the early stage of bone healing of mandibular critical bone defect in dogs. Because the hydroxysterol bone mass has good spatial maintenance ability, it is more suitable for the repair of large area bone defect in clinic.
【作者单位】： 南方医科大学口腔医院(广东省口腔医院);
【基金】：国家自然科学基金(No:81170998) 广东省医学科研基金(No:C2012034)
【分类号】：R782.2
，
本文编号：2508760
[Abstract]:Objective to compare the osteogenic effects of compound hydroxysterol inorganic calf bone mass (hydroxysterol bone mass) and inorganic calf bone powder in the early healing stage of mandibular critical bone defect in Beagle dogs. Methods four healthy male Beagle dogs were selected. After bilateral mandibular third and fourth premolars and first molar (P3-M1) were removed, two 5 mm 脳 10 mm standardized box critical bone defects were replicated in each side of the jaw. After 4 weeks of healing, the hydroxysterol bone mass and inorganic calf bone powder were randomly implanted into bone defects according to half mouth control design. Four weeks after operation, the specimens were cut to make paraffin and hard tissue sections, and the histology and histomorphology were observed. Results the experimental animals healed well. There were obvious osteoblasts and new bone formation at the edge of critical bone defect and the junction of bone material and host bone in the hydroxysterol bone mass group and the inorganic calf bone powder group. The histomorphology of HE staining showed that the ratio of new bone area in the hydroxysterol bone mass group and the inorganic calf bone powder group was (37.22 卤2.16)% and (34.70 卤2.66)%, respectively, and there was no significant difference between the two groups (P 0.05). The number of bone cells composed of hydroxysterol bone mass [(31.13 卤2.85)] was significantly different from that of inorganic calf bone powder group [(24.88 卤2.95)] (P 0.05). The relative percentage of new bone area of hydroxysterol bone mass and inorganic calf bone powder was (20.13 卤1.32)% and (19.28 卤0.82)%, respectively, and the relative retention rate of bone material was (30.80 卤1.16)% and (29.69 卤1.12)%, respectively. there was no significant difference between the two groups (P 0.05). The results showed that the relative percentage of new bone area was (20.13 卤1.32)% and (19.28 卤0.82)%, and the relative retention rate of bone material was (30.80 卤1.16)% and (29.69 卤1.12)%, respectively. Conclusion the osteogenic effect of hydroxysterol bone mass is similar to that of calf bone powder in the early stage of bone healing of mandibular critical bone defect in dogs. Because the hydroxysterol bone mass has good spatial maintenance ability, it is more suitable for the repair of large area bone defect in clinic.
【作者单位】： 南方医科大学口腔医院(广东省口腔医院);
【基金】：国家自然科学基金(No:81170998) 广东省医学科研基金(No:C2012034)
【分类号】：R782.2
，
本文编号：2508760
本文链接：https://www.wllwen.com/yixuelunwen/kouq/2508760.html
最近更新
教材专著
