RGD肽修饰壳聚糖作为种植体表面基因载体的研究

发布时间：2019-07-02 13:49

【摘要】：目的探索改善钛种植体表面处理的新方法,提高种植体植入后成骨效率。方法将RGD肽与壳聚糖(CS)通过酰化反应发生偶联形成RGD-CS,采用复凝聚法制备RGD-CS/pDNA复合体,并将RGD-CS/pDNA复合体接枝到经物理、生化处理后的纯钛表面。采用红外光谱仪和元素分析仪对RGD-CS进行化学结构的表征检测,凝胶电泳阻滞试验结合原子力显微镜观察RGD-CS对质粒的包裹情况及RGD-CS/pDNA复合体的形态,EB染色法检测钛片表面RGD-CS/pDNA复合体的接枝效果。结果红外光谱检测结合元素分析表明,CS和RGD肽偶联成功;凝胶电泳阻滞试验结合原子力显微镜观察表明,在N/P≥2时,RGD-CS与pDNA完全复合,RGD-CS/pDNA复合体呈类球形;EB染色表明RGD-CS/pDNA复合体接枝钛片成功。结论经RGD肽修饰的壳聚糖可以携带pDNA作为钛种植体表面质粒包装的载体。
[Abstract]:Objective to explore a new method to improve the surface treatment of titanium implantation and to improve the osteogenic efficiency after implantation. Methods RGD-CS/pDNA complex was prepared by coupling RGD peptide with chitosan (CS) by acylation to form RGD-CS/pDNA by complex coagulation method, and RGD-CS/pDNA complex was graft onto pure titanium surface after physical and biochemical treatment. The chemical structure of RGD-CS was characterized by infrared spectrometer and element analyzer. The encapsulation of plasmid by RGD-CS and the morphology of RGD-CS/pDNA complex were observed by gel electrophoresis block test combined with atomic force microscope. The grafting effect of RGD-CS/pDNA complex on titanium sheet was detected by EB staining. Results Infrared spectroscopy combined with elemental analysis showed that the coupling of CS and RGD peptides was successful, gel electrophoresis block test combined with atomic force microscope showed that when N 鈮，

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