miR-144在人牙周膜成纤维细胞中调控Toll样受体2及骨唾液酸蛋白的表达

发布时间：2021-02-24 17:30

　　目的检测与Toll样受体2（Toll-like receptor 2,TLR2）呈负相关性的微小RNA（microRNA,miRNA）在人牙周膜成纤维细胞（human periodontal ligament fibroblasts,hPDLFs）中与TLR2的作用关系及其对骨唾液酸蛋白（bone sialoprotein,BSP）的调控作用。方法查找与TLR2具有靶向结合关系且呈负相关性的miRNA（miR-23a、miR-144、miR-19a）, Real-time PCR方法检测正常及牙龈卟啉单胞菌（Porphyromonas gingivals,P.gingivalis）胞壁成分脂多糖（lipopolysaccharide,LPS）诱导下hPDLFs中目标miRNA的mRNA表达水平;分空白对照组、阴性对照（LPS）组、miRNA过表达对照组、miRNA过表达组、miRNA抑制对照组、miRNA抑制组,用Lipofectamine 2000分别转染hPDLFs,Real-time PCR及Western Blot法分别测定TLR2、BSP的mRNA及蛋白表达水平。结果经LPS... 

【文章来源】：口腔医学. 2020,40(07)

【文章页数】：6 页

【部分图文】：

第4代hPDLFs(倒置显微镜 ×40)

LPS诱导hPDLFs后TLR2的表达

正常及LPS诱导下hPDLFs中miRNA的表达

【参考文献】：
期刊论文
[1]MicroRNAs and immunity in periodontal health and disease[J]. Xianghong Luan,Xiaofeng Zhou,Afsar Naqvi,Marybeth Francis,Deborah Foyle,Salvador Nares,Thomas G.H.Diekwisch.  International Journal of Oral Science. 2018(03)
[2]微小RNAs在牙周致病过程中的调控机制研究进展[J]. 姜慕舟,黄婉怡,林莉.  中国实用口腔科杂志. 2017(01)
[3]Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues[J]. Yu-feng Xie1, Rong Shu1*, Shao-yun Jiang1, Da-li Liu1, Xiu-li Zhang2 1Department of Periodontology, Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2Shanghai Research Institute of Stomatology, Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.  International Journal of Oral Science. 2011(03)



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