老挝南塔省芒新县蚊虫种类及其带毒率调查

发布时间：2018-03-25 16:08

本文选题：蚊种组成　切入点：诱蚊灯法　出处：《大理大学》2017年硕士论文

【摘要】：【研究目的】调查和弄清老挝南塔省芒新县蚊虫种类及其重要虫媒病毒带毒率情况,为制定当地重要蚊虫病毒性疾病防控对策与措施提供重要依据。【研究方法】采用诱蚊灯通宵捕蚊法采集成蚊,并采用幼虫捕捞法对树洞、竹筒、石穴IV龄幼虫进行采集,采集的蚊虫或IV幼虫采用形态学方法鉴定蚊虫种类;采用白纹伊蚊C6/36细胞接种培养和采用RT-PCR方法进行病毒分离鉴定,计算出重要虫媒病毒带毒率。【结果】共采获老挝南塔省芒新县蚊虫21781只、11属44种,其中在所采集的成蚊种类中,三带喙库蚊和中华按蚊为当地的优势蚊虫种类,分别占捕获成蚊总数的54.4%(11600/21325)和39.28%(8377/21325);IV龄幼虫,黄色阿蚊和斑翅库蚊所占比例较高,分别占捕获IV龄幼虫总数的43.42%(198/456)和18.86%(86/456)。从现场采集的90组蚊虫样本中,三带喙库蚊中分离出了14株蚊传黄病毒和4株版纳病毒,从中华按蚊中分离出3株版纳病毒;三带喙库蚊传黄病毒和版纳病毒的批阳性率分别为15.56%(14/90)和7.78%(7/90),中华按蚊版纳病毒批阳性率为42.86%(3/7)。【结论】老挝芒新县蚊虫种类丰富,三带喙库蚊和中华按蚊属于当地成蚊优势蚊种,从三带喙库蚊中分离出14株蚊传黄病毒和4株版纳病毒,从中华按蚊中分离出3株版纳病毒,蚊传黄病毒和版纳病毒的批阳性率分别为15.56%(14/90)和7.78%(7/90),为当地卫生部门应加强虫媒媒介及其疑似病例监测提供依据。
[Abstract]:[objective] to investigate and clarify the species of mosquitoes and the prevalence of important insect-borne viruses in Mengxin County, South Ta Province, Laos. In order to provide important basis for the prevention and control of local important mosquito virus diseases, the mosquito adult was collected by mosquito trap overnight, and the IV instar larvae of tree hole, bamboo tube and stone hole were collected by larval fishing method. The collected mosquitoes or IV larvae were identified by morphological method, the Aedes albopictus C6 / 36 cells were inoculated and cultured, and the virus was isolated and identified by RT-PCR method. [results] A total of 21781 mosquitoes belonging to 11 genera and 44 species were collected from Mengxin County, South Ta Province, Laos. Among them, Culex tritaeniorhynchus and Anopheles sinensis were the dominant species of mosquitoes in the area, among which Culex tritaeniorhynchus and Anopheles sinensis were the dominant species. They accounted for 54.400 / 21325% of the total number of captured adult mosquitoes, and 39.28% 8377 / 21325% IV instar larvae, 43.42% (43.42%) and 18.86 8686% 456% of the total number of captured IV instar larvae, respectively. From 90 mosquito samples collected from the field, the proportion of them was higher than that of Culex albopictus and Culex pipiens albopictus. 14 strains of mosquito yellow virus and 4 strains of Banna virus were isolated from Culex tritaeniorhynchus and 3 strains of Banna virus were isolated from Anopheles sinensis. The positive rates of Anopheles sinensis and Anopheles sinensis were 15.5614 / 90 and 7.78 / 70 / 90, respectively. [conclusion] there are abundant species of mosquitoes in Mengxin County, Laos, and the three Culex rotunda and Anopheles sinensis belong to the dominant species of adult mosquitoes. 14 strains of mosquito yellow virus and 4 strains of Banna virus were isolated from Culex tritaeniorhynchus and 3 strains of Banna virus were isolated from Anopheles sinensis. The positive rates of mosquito transmitted yellow virus and Banna virus were 15.5614 / 90) and 7.78 / 90 / 90 respectively, which provided the basis for the local health department to strengthen the surveillance of insect-vector and its suspected cases.
【学位授予单位】：大理大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R184

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