解脲支原体在健康幼儿泌尿生殖道中的分子流行病学研究

发布时间：2018-03-29 11:34

本文选题：幼儿　切入点：解脲支原体　出处：《青岛大学》2007年硕士论文

【摘要】： 目的：研究解脲支原体(Ureaplasma urealyticum，Uu)在健康幼儿泌尿生殖道中的检出情况与分群分型，以探讨Uu在该人群泌尿生殖道中的分子流行病学情况。方法：收集健康幼儿尿液400例，将离心后的尿沉渣直接接种于解脲支原体液体选择性培养基进行初筛，初筛阳性的标本用PCR法进行分群。对Uu一群的标本再用PCR法及酶切法进一步分型。采用SPSS12.5统计软件包进行统计学分析。结果：400例健康幼儿中Uu液体培养阳性36例，PCR扩增阳性32例(8.00％)，4例未扩增。其中单纯生物一群23例(71.88％)，单纯生物二群7例(21.88％)，两群混合2例(6.24％)。生物一群中1型7例(21.88％)，6型11例(34.38％)，3／14型4例(12.50％)，混合型1例(3.12％)。252例男婴中阳性12例(4.76％)，148例女婴中阳性20例(13.51％)，两组阳性率差别有统计学意义(x~2=9.70，，P＜0.01)。结论：解脲支原体在健康幼儿泌尿生殖道中的携带率为8.00％，分离的Uu以生物一群为主；女性幼儿Uu检出率高于男性幼儿；Uu可能属于人体的一种共生微生物。
[Abstract]:Objective: to study the detection and classification of Ureaplasma Ureaplasma Ureaplasma Ure@@. Methods: the urine samples of 400 healthy children were collected, and the urine sediment after centrifugation was inoculated directly into Ureaplasma Urealyticum liquid selective medium for primary screening. The positive samples were divided into groups by PCR method. The samples of UU group were further classified by PCR method and enzyme digestion method. The statistical analysis was carried out by SPSS12.5 statistical software package. Results among the 400 healthy children, 36 cases were positive for UU liquid culture, 32 cases were positive for PCR amplification, and 4 cases were not amplified. Among them, 23 cases were simple biological group (23 cases), 7 cases were simple biological group 2 (21.88%), 2 cases were mixed group (2 cases), 2 cases were mixed with two groups, 7 cases were type 1 in biological group, 7 cases were type 21.88. 11 cases (34.38%) were divided into 3 / 14 type (n = 4), 12.50% (n = 4), 1 case of mixed type (n = 1), 3.12% (n = 2). Among 252 male infants, 12 cases were positive (n = 12) and 20 cases (n = 20) were positive in 148 female infants. The difference between the two groups was statistically significant (P < 0.01). Conclusion: the Ureaplasma Urealyticum carrying rate in the urogenital tract of healthy children is 8.00, the isolated UU is mainly biological group, the detection rate of Ureaplasma Urealyticum in female children is higher than that in male infants, and the Ureaplasma Urealyticum may be a symbiotic microorganism in human body.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R181.3

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