过氧化氢低温等离子体灭菌效果及影响因素研究

发布时间：2018-04-12 22:27

  本文选题：载体定性培养方法 + 过氧化氢低温等离子体　； 参考：《中国消毒学杂志》2014年09期

【摘要】：目的研究过氧化氢低温等离子体灭菌效果及影响因素,为科学使用提供参考。方法采用载体定性培养方法,对不同因素影响低温等离子体灭菌器的灭菌效果进行实验室研究。结果在常规的灭菌运行程序条件下,将染菌载体置于孔径1 mm、长度1 000 mm的管腔内,30份枯草杆菌黑色变种芽孢全部达到灭菌,30份嗜热脂肪杆菌芽孢中有23份达到灭菌。在孔径1 mm,长度为150 mm和1 000 mm的管腔内放置嗜热脂肪杆菌芽孢染菌载体,经常规灭菌程序处理,150 mm管腔内30份染菌载体中24个达到灭菌,1 000 mm管腔中只有10个达到灭菌。分别在两端相通和一端盲管的150 mm管腔内各放30份嗜热脂肪杆菌芽孢染菌载体,结果相通管腔内23份达到灭菌,盲管内14个达到灭菌;1 mm孔径管腔内14个达到灭菌,2 mm孔径的管腔内有23个达到灭菌。结论嗜热脂肪杆菌芽孢对过氧化氢低温等离子体抗力强于枯草杆菌黑色变种芽孢;管腔孔径和长度影响低温等离子体灭菌效果。
[Abstract]:Objective to study the sterilization effect and influencing factors of hydrogen peroxide low temperature plasma, and to provide reference for scientific application.Methods the effect of different factors on the sterilizing effect of low temperature plasma sterilizer was studied by carrier qualitative culture method.Results under the normal sterilization procedure, the carrier was placed in the pore diameter of 1 mm and the length of 1 000 mm in the tube cavity. All 30 spores of Bacillus subtilis var. Niger were sterilized and 23 of the 30 spores of adipose thermophilic bacteria reached sterilization.In the tube with diameter of 1 mm, length of 150 mm and 1 000 mm, the carrier of Bacillus thermophilus spores was placed in the tube cavity. Only 10 of the 30 carriers in 150 mm tube cavity were sterilized by routine sterilization procedure.In 150 mm tube with both ends connected and one end blind tube, 30 spores carriers of adipobacter thermophilus were put into each other, and 23 of them were sterilized in the same tube.In 14 cavities with sterilizing diameter of 1 mm in diameter, 23 reached sterilization in 14 cavities with a diameter of 2 mm in the blind tube.Conclusion the resistance to hydrogen peroxide low temperature plasma is stronger than that of Bacillus subtilis var. Niger, and the diameter and length of tube cavity affect the sterilizing effect of low temperature plasma.
【作者单位】： 江苏省疾病预防控制中心;
【基金】：“十二五”科教兴卫工程“突发公共卫生事件应急处置创新平台”(ZX201109) 空气中结核菌的分离采集及消毒干预措施的建立(Y2012086)
【分类号】：R187
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本文编号：1741715