离子色谱法检测Y群和W135群脑膜炎球菌荚膜多糖含量

发布时间：2018-04-14 12:34

  本文选题：高效阴离子交换色谱-脉冲安培检测法 + 多糖疫苗　； 参考：《中国新药杂志》2014年15期

【摘要】：目的:建立Y群W135群脑膜炎球菌荚膜多糖含量的高效阴离子交换色谱-脉冲安培检测法(high-performance anion-exchange chromatography with pulsed amperometric detection,HPAEC-PAD),并对该方法进行验证。方法:通过对水解温度、水解时间和水解用酸及其浓度的筛选,确定最佳的水解条件;利用固总法和化学法衡量HPAEC-PAD法的准确性,检测ACYW135群脑膜炎球菌多糖疫苗和Y群W135群多糖蛋白结合疫苗原液,评价其重复性和精密性。结果:最佳水解条件为2mol·L-1 HCL 80℃水解1h。色谱法的检测结果与固总法和化学法比较,CV在1.60%~5.04%,结果准确。检测Y群和W135群多糖含量重复性和精密性均良好。结论:HPAEC-PAD法可以用于Y群W135群多糖含量的测定。
[Abstract]:Aim: to establish a high performance anion-exchange chromatography with pulsed amperometric detection method for high performance anion-exchange chromatography with pulsed amperometric detection of meningococcal meningococcal capsule polysaccharides (HPAEC-PADX) in group Y, and to verify the method.Methods: the optimum hydrolysis conditions were determined by screening hydrolysis temperature, hydrolysis time, hydrolysis acid and its concentration, and the accuracy of HPAEC-PAD method was measured by solid sum method and chemical method.ACYW135 meningococcal polysaccharide vaccine and Y group W135 polysaccharide protein binding vaccine were detected to evaluate their reproducibility and accuracy.Results: the optimum hydrolysis condition was 2mol L-1 HCL for 1 h at 80 鈩，

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