钢铁厂冷却塔水嗜肺军团菌分离株分子流行病学研究

发布时间：2018-04-21 23:36

本文选题：嗜肺军团菌 + 毒力基因　；参考：《环境与健康杂志》2017年08期

【摘要】：目的调查某钢铁企业冷却塔水嗜肺军团菌的污染状况及分子流行病学特征,为预防控制军团菌病的传播提供科学依据。方法 2011年3月—2012年9月,对石家庄市某钢铁厂车间冷却塔水进行监测,分离培养嗜肺军团菌,对得到的菌株进行血清分型、毒力基因检测(lvh、rtxA)和多位点序列分型研究。结果 182份冷却塔水中有67份检出嗜肺军团菌,检出率为36.8%;共分离培养得到菌株84株,包括7种血清型,其中LP1型占63.1%(53/84)、LP6型占19.0%(16/84)。lvh和rtxA基因检测阳性的分别有79株和83株,二者均为阳性的有78株。多位点序列分型可分为32个型,SBT1型占36.9%(31/84),为主要优势型别,有21个型别为首次发现。结论钢铁企业冷却水存在嗜肺军团菌污染,携带有毒力基因,血清型和SBT型呈多样性分布。
[Abstract]:Objective to investigate the contamination status and molecular epidemiological characteristics of Legionella pneumophila in cooling tower of a steel enterprise, and to provide scientific basis for preventing and controlling the spread of Legionella. Methods from March 2011 to September 2012, the cooling tower water of a steel plant in Shijiazhuang City was monitored, and the strains of Legionella pneumophila were isolated and cultured. The serotyping, virulence gene detection and multiple loci sequence typing were studied. Results Sixty-seven strains of Legionella pneumophila were detected in 182 samples of cooling tower water, and 84 strains were isolated and cultured, including 7 serotypes, of which 79 strains were positive for 19.0%(16/84).lvh and 83 strains for rtxA gene for LP1 type. 78 strains were positive for both. The multilocus sequence typing can be divided into 32 types of SBT1 type, 36.9% of which is the dominant type, 21 of which are found for the first time. Conclusion there is Legionella pneumophila contamination in cooling water of iron and steel enterprises, which contains toxic gene, serotype and SBT type.
【作者单位】：石家庄市疾病预防控制中心微生物检验所;上海市杨浦区疾病预防控制中心实验室;
【基金】：石家庄市科学技术研究与发展指导计划课题(12146953)
【分类号】：R181.3;R517.9

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