云南省2011-2014年急性弛缓性麻痹病例非脊髓灰质炎肠道病毒分子流行病学特征

发布时间：2018-04-22 05:17

本文选题：急性弛缓性麻痹 + 非脊髓灰质炎肠道病毒　；参考：《中国疫苗和免疫》2017年06期

【摘要】：目的了解非脊髓灰质炎(脊灰)肠道病毒(Non-polio enterovirus,NPEV)的型别分布及分子进化特征。方法对2011-2014年从云南省急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例中分离到的148株NPEV进行VP1完整编码区核苷酸的扩增与序列测定。所获得的云南地方株基因序列与各基因型原型株进行核苷酸与氨基酸同源性比较,并与Gen Bank中选取的代表株构建系统进化树。结果在148株NPEV分离株中,肠道病毒(Enterovirus,EV)A组(EV-A)、EV-B、EV-C分别占31.1%(46株,8个血清型)、64.9%(96株,27个血清型)、4.1%(6株,3个血清型);基因进化树显示,各种血清型病毒株与对应原型株及代表株聚集;除埃可病毒10型(E-10)和EV-C96型外,云南地方株与原型株位于不同分支。在4年的流行过程中,同一个型别的分离株之间的遗传变异程度最高的是EV-C96、E-18和E-5,分别达到21.5%、21.3%和20.4%。结论云南省存在EV多个型别的循环,需持续性监测和探索EV分子流行病学和进化规律。
[Abstract]:Objective to investigate the type distribution and molecular evolution of non polioenterovirusNPEV (Non-polio enterovirusNPEV). Methods 148 strains of NPEV isolated from acute flaccid paralysis in Yunnan province from 2011 to 2014 were amplified and sequenced in the complete coding region of VP1. The nucleotide and amino acid homology of Yunnan native strain was compared with that of each genotypic prototype strain, and the phylogenetic tree was constructed with the representative strain selected in Gen Bank. Results among the 148 NPEV isolates, the Enterovirus-EVV EV-C group accounted for 31.1%, 8 serotypes 64.9%, 27 serotypes 6 strains and 3 serotypes, and the phylogenetic tree showed that all serotypes, their corresponding prototypes and their representative strains were clustered. With the exception of Eco virus type 10 (E-10) and EV-C96, Yunnan native strain and prototype strain are located in different branches. During the 4 years of epidemic, the highest degree of genetic variation among isolates of the same type was EV-C96 E-18 and E-5, which reached 21.521.3% and 20.4g, respectively. Conclusion there are several types of EV cycles in Yunnan Province, and it is necessary to continuously monitor and explore the molecular epidemiology and evolution of EV.
【作者单位】：云南省疾病预防控制中心云南省肠道病毒研究中心;
【基金】：国家自然科学基金云南省肠道病毒分子生物学特征及监测研究(编号:81160198)
【分类号】：R181.3;R512.4

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