氯己定乙醇与碘伏对MRSA体外抗菌活性研究

发布时间：2018-04-24 13:26

本文选题：醋酸氯己定乙醇 + 碘伏　；参考：《中国消毒学杂志》2014年08期

【摘要】：目的观察氯己定乙醇与碘伏对医院分离的26株耐甲氧西林金黄色葡萄球菌(MRSA)和标准菌株金黄色葡萄球菌(ATCC 29213)的体外抗菌活性。方法采用经典二倍稀释法,进行最低抑菌浓度(MIC)试验和最低杀菌浓度(MBC)试验。结果两种消毒剂对26株MRSA的MIC值均等于标准菌株(分别为1 mg/L醋酸氯己定乙醇、21 mg/L碘伏),两种消毒剂对其中23株MRSA的MBC值等同于MIC值。醋酸氯己定乙醇对2株MRSA的MBC值为标准菌株的31倍;碘伏对2株MRSA的MBC值为标准菌株的2倍;其中1株MRSA对醋酸氯己定乙醇的MBC值为标准菌株的31倍,同时对碘伏的MBC值为标准菌株的2倍。结论对醋酸氯己定乙醇及碘伏均产生抗性的MRSA菌株已经出现,且具有交叉耐药性。应积极探讨高效低毒消毒剂的研制与合理应用、消除细菌对消毒剂抗性的问题。
[Abstract]:Objective to observe the in vitro antibacterial activity of chlorhexidine ethanol and iodophor against 26 strains of methicillin-resistant Staphylococcus aureus (MRSAA) and a standard strain of Staphylococcus aureus (ATCC29213). Methods the minimal inhibitory concentration (MIC) test and the minimum bactericidal concentration (MBC) test were performed by the classical double dilution method. Results the MIC values of the two disinfectants to 26 MRSA strains were equal to those of the standard strains (1 mg/L chlorhexidine acetate alcohol, 21 mg/L iodophor), and the MBC values of the two disinfectants to 23 MRSA strains were equal to the MIC value. The MBC value of chlorhexidine acetate to 2 strains of MRSA was 31 times of that of the standard strain, the MBC value of iodophor to 2 strains of MRSA was 2 times that of the standard strain, and the MBC value of 1 MRSA to chlorhexidine acetate ethanol was 31 times of that of the standard strain. At the same time, the MBC value of iodophor was twice as high as that of the standard strain. Conclusion MRSA strains with resistance to chlorhexidine acetate ethanol and iodophor have been found and have cross resistance. The development and rational application of high efficiency and low toxicity disinfectants should be actively discussed to eliminate the problem of bacterial resistance to disinfectants.
【作者单位】：重庆医科大学附属第一医院;
【分类号】：R187

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