2010~2011年广州献血人群HIV-1流行病学分析

发布时间：2018-05-01 04:37

本文选题：HIV- + 亚型　；参考：《中国输血杂志》2014年03期

【摘要】：目的了解广州地区献血人群中艾滋病病毒1型(HIV-1)感染的流行病学情况。方法对广州地区581656例无偿献血者标本用2个不同厂家的ELISA法试剂同时检测抗-HIV,双试剂阳性者送广州市疾控中心用蛋白免疫印迹试验(WB)进行确认,从WB法阳性的献血者的血浆中提取核酸,用RT-PCR进行gag区基因扩增,对扩增产物进行测序和序列分析,并进行基因分型,用spss16.0软件对流行病学资料进行统计。结果 ELISA法抗-HIV双试剂阳性133例(0.023%,133/581 656),WB法检测120例HIV-1抗体阳性(0.021%,120/581 656),不确定13例,120例WB法阳性的献血者中有6种亚型:CRF01-AE重组亚型51例;CRF07-BC重组亚型32例;CRF08-BC重组亚型15例;01B型16例;B'型6例,通过异性性传播组的阳性率高于其他途径传播组,男性组高于女性组,外地身份证的献血者高于广州市身份证者,首次献血者高于再次献血者,文化程度为初中及以下者高于其他组,年龄26~35岁组高于其他年龄组。结论本地区献血人群中HIV-1感染者以外地男性青壮年为主,主要通过性传播。
[Abstract]:Objective to investigate the epidemiology of HIV-1 infection in blood donors in Guangzhou. Methods 581656 volunteer blood donors from Guangzhou area were tested for anti-HIVs with ELISA reagent from two different manufacturers, and those with positive double reagents were sent to the Center for Disease Control and Prevention of Guangzhou by Western blotting test. The nucleic acid was extracted from the plasma of the blood donors with WB method, the gag region gene was amplified by RT-PCR, the amplified products were sequenced and sequenced, the genotyping was carried out, and the epidemiological data were analyzed by spss16.0 software. Results in 133 cases of anti-HIV double reagents positive by ELISA method, 0.023% 581 656A WB method was used to detect 120 cases of HIV-1 antibody positive, 0.021% 0.021%, 581 656%, and 6 kinds of CRF01-AE recombinant subtype 51 cases of CRF07-BC recombinant subtype 32 cases and 15 cases of CRF08-BC recombinant subtype were detected. There were 16 cases of type B', 6 cases of type B'. The positive rate of heterosexual transmission group was higher than that of other transmission group, the male group was higher than the female group, the blood donor of the field identity card was higher than the Guangzhou identity card, the first blood donor was higher than the second donor. The education level was higher in the junior middle school group than in the other groups, and the age group was higher in the age group of 26 to 35 years than that of the other age groups. Conclusion HIV-1 infection in blood donors in this area is mainly transmitted by sexual transmission among male adults in other areas.
【作者单位】：广州血液中心检验科;
【分类号】：R181.3

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