甲型H1N1流感病毒流行病学调查

发布时间：2018-05-07 01:05

本文选题：甲型HN流感病毒 + 甲型HN特异性抗体　；参考：《中国免疫学杂志》2014年08期

【摘要】：目的:了解甲型H1N1流感病毒的流行特点及传播规律,加强对流感大流行的防控。方法:收集2009年10~12月吉林大学第一医院住院发热门诊中具有流感症状患者的临床样品(鼻咽拭子和血清),采用血凝抑制法、巢式RT-PCR技术检测具有流感症状患者血清标本中的特异性抗体、咽拭子中的甲型H1N1 RNA等指标。结果:结果 56份流感患者的咽拭子经核酸检测,甲型流感NP和M的阳性率分别为37.5%和27.8%,而甲型H1N1的阳性率仅为3.6%。通过血凝抑制实验结果表明:在相应的血清标本中,能特异性抑制甲型H1N1流感病毒红细胞凝集的样品为27份,抗体均大于1∶320,其中8份样品抗体效价大于1∶5 120。恢复期的血清效价与急性期相比有显著的抗体差异,甲型H1N1病毒特异性抗体阳性率为48.2%,明显高于核酸的检测。结论:核酸检测可在感染早期(排毒期)进行检测,而血清学的抗体检测多用在感染中后期(抗体产生)检测,两者联合可提高甲型H1N1流感的检出率。
[Abstract]:Objective: to understand the epidemic characteristics and transmission rules of influenza A H1N1 virus and to strengthen the prevention and control of influenza pandemic. Methods: the clinical samples (nasal swabs and serums) were collected from inpatients with fever in the first Hospital of Jilin University from October to December 2009. Hemagglutination inhibition method was used. Nested RT-PCR technique was used to detect the specific antibodies in serum samples of patients with influenza symptoms and the A H1N1 RNA in throat swabs. Results: the positive rates of NP and M of influenza A were 37.5% and 27.8%, respectively, while the positive rate of A H1N1 was only 3.6%. The results of hemagglutination inhibition test showed that in the corresponding serum samples, 27 samples could specifically inhibit the agglutination of influenza A (H1N1) virus erythrocytes, and all the antibodies were more than 1: 3200.The titers of 8 samples were more than 1:5. The titer of serum in convalescent stage was significantly higher than that in acute stage. The positive rate of specific antibody against A H1N1 virus was 48.2, which was significantly higher than that of nucleic acid. Conclusion: nucleic acid detection can be detected in the early stage of infection (detoxification stage), while serological antibody detection can be used in the middle and late stage of infection (antibody production), combined with both can improve the detection rate of influenza A H1N1.
【作者单位】：吉林大学第一医院感染科;军事医学科学院军事兽医研究所;
【基金】：国家自然科学基金资助项目(No.31072124和No.31030064)
【分类号】：R181.3;R511.7

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