1966-2011年全球肠道病毒71型部分分离株的基因特征及流行病学分析

发布时间：2018-05-08 00:03

本文选题：肠道病毒型(EV) + VP　；参考：《现代预防医学》2014年07期

【摘要】：目的分析人类肠道病毒71型(EV71)基因特征及流行病学信息。方法用生物信息学软件对来自13个国家、中国的17省、直辖市不同时间内分离的270株EV71病毒VP1区基因序列进行分析,计算不同毒株的核苷酸差异率以及基因型/亚型内部、之间的同源性,构建系统发生树,分析流行病学信息。结果 270株病毒可分为3个基因型(A、B、C型),B型和C型又各可分为5个亚型;其中A型只有3株,B型和C型占了98.9%。不同毒株的核苷酸差异率为7.1%~25.9%,不同基因型之间的核苷酸差异率为23.0%~25.9%。同时从发生树中发现一些区别于B型和C型的"孤儿株"。结论同一地区可以出现某种基因型/亚型流行很长时间,同一时间内可以出现不同的基因型/亚型在某地区流行,具有明显的时间和地域流行特点。"孤儿株"的发现为EV71的不断进化以及具有基因多态性提供了证据。
[Abstract]:Objective to analyze the gene characteristics and epidemiological information of human enterovirus 71 EV71. Methods the VP1 gene sequences of 270 EV71 virus strains isolated from 13 countries, 17 provinces and municipalities of China were analyzed by bioinformatics software. The nucleotide difference rates and genotypes / subtypes of different strains were calculated. A phylogenetic tree was constructed and epidemiological information was analyzed. Results 270 strains could be divided into three genotypes: Agna B and C, respectively, and only 3 strains of A accounted for 98.9% of them. The nucleotide difference rate of different strains was 7.1and 25.9.The difference rate of nucleotide between different genotypes was 23.0and 25.9. At the same time, we found some "orphan plants" which are different from B type and C type from the phylogenetic tree. Conclusion A certain genotype / subtype epidemic can occur in the same area for a long time, and different genotypes / subtypes may occur in a certain area at the same time. The discovery of orphan strains provides evidence for the continued evolution and genetic polymorphism of EV71.
【作者单位】：南方医科大学公共卫生与热带医学学院三级生物安全实验室;
【基金】：广东省科技计划资助项目:生物医药高通量数据分析服务平台建设(2010A040302003)
【分类号】：R181.39

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